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CENTRAL DOGMA & STRUCTURE-FUNCTION RELATIONSHIPS IN PROTEINS You are a bacteriologist studying a pathogenic protein (the "BAD" protein) that contributes to diseases caused by Staphylococcus aureus. BAD functions as an aß heterodimer, and the a subunit (25KD) and B subunit (75KD) are held together by an electrostatic interaction between K in the a subunit and D in the B subunit. You are trying to dissociate the BAD subunits to prevent pathogenesis in the bacteria. Which mutation would you make to prevent the BAD subunits from forming a dimer? Assume neutral pH. (A) introduce AAA → AGA point mutation in the a subunit gene (B) introduce GAC → GAG point mutation in the ß subunit gene (C) introduce GAT → CGT point mutation in the B subunit gene (D) introduce two point mutations: AAG → GTG in the a subunit gene & GÁC → CTC in the B subunit gene (E) introduce two point mutations: AAA → TGC in the a subunit gene & GAT → TGT in the B subunit gene

CENTRAL DOGMA & STRUCTURE-FUNCTION RELATIONSHIPS IN PROTEINS You are a bacteriologist studying a pathogenic protein (the "BAD" protein) that contributes to diseases caused by Staphylococcus aureus. BAD functions as an aß heterodimer, and the a subunit (25KD) and B subunit (75KD) are held together by an electrostatic interaction between K in the a subunit and D in the B subunit. You are trying to dissociate the BAD subunits to prevent pathogenesis in the bacteria. Which mutation would you make to prevent the BAD subunits from forming a dimer? Assume neutral pH. (A) introduce AAA → AGA point mutation in the a subunit gene (B) introduce GAC → GAG point mutation in the ß subunit gene (C) introduce GAT → CGT point mutation in the B subunit gene (D) introduce two point mutations: AAG → GTG in the a subunit gene & GÁC → CTC in the B subunit gene (E) introduce two point mutations: AAA → TGC in the a subunit gene & GAT → TGT in the B subunit gene

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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CENTRAL DOGMA & STRUCTURE-FUNCTION RELATIONSHIPS IN PROTEINS You are a bacteriologist studying a pathogenic protein (the "BAD" protein) that contributes to diseases caused by Staphylococcus aureus. BAD functions as an aß heterodimer, and the a subunit (25kD) and ß subunit (75KD) are held together by an electrostatic interaction between K in the a subunit and D in the B subunit. You are trying to dissociate the BAD subunits to prevent pathogenesis in the bacteria. Which mutation would you make to prevent the BAD subunits from forming a dimer? Assume neutral pH. (A) introduce AAA → AGA point mutation in the a subunit gene (B) introduce GAC → GAG point mutation in the ß subunit gene (C) introduce GAT → CGT point mutation in the ß subunit gene (D) introduce two point mutations: AAG → GTG in the a subunit gene & GAC → CTC in the B subunit gene (E) introduce two point mutations: AAA → TGC in the a subunit gene & GAT → TGT in the B subunit gene second base in codon A TGT Cys TGC Cys TTT Phe ТAT Tyr TAC Tyr TAA stop TCT Ser T TTC Phe TCC Ser TTA Leu TCA Ser TGA stop A TTG Leu TCG Ser TAG stop TGG Trp ССT Pro CCC Pro CCA Pro CCG Pro CGT Arg CGC Arg CGA Arg CGG Arg CTT Leu CAT His T CTC Leu CAC His CTA Leu CAA Gin A CTG Leu CAG GIn ATT lle АСT Thr AAT Asn AGT Ser ATC lle ACC Thr AAC Asn AGC Ser A ATA lle AAA Lys AAG Lys AGA Arg AGG Arg ACA Thr A ATG Met ACG Thr GAT Asp GAC Asp GGT Gly GGC Gly GGA Gly GTT Val GCT Ala GTC Val GCC Ala G GTA Val GCA Ala GAA Glu A GTG Val GCG Ala GAG Glu GGG Gly G third base in codon first base in codon
Transcribed Image Text:CENTRAL DOGMA & STRUCTURE-FUNCTION RELATIONSHIPS IN PROTEINS You are a bacteriologist studying a pathogenic protein (the "BAD" protein) that contributes to diseases caused by Staphylococcus aureus. BAD functions as an aß heterodimer, and the a subunit (25kD) and ß subunit (75KD) are held together by an electrostatic interaction between K in the a subunit and D in the B subunit. You are trying to dissociate the BAD subunits to prevent pathogenesis in the bacteria. Which mutation would you make to prevent the BAD subunits from forming a dimer? Assume neutral pH. (A) introduce AAA → AGA point mutation in the a subunit gene (B) introduce GAC → GAG point mutation in the ß subunit gene (C) introduce GAT → CGT point mutation in the ß subunit gene (D) introduce two point mutations: AAG → GTG in the a subunit gene & GAC → CTC in the B subunit gene (E) introduce two point mutations: AAA → TGC in the a subunit gene & GAT → TGT in the B subunit gene second base in codon A TGT Cys TGC Cys TTT Phe ТAT Tyr TAC Tyr TAA stop TCT Ser T TTC Phe TCC Ser TTA Leu TCA Ser TGA stop A TTG Leu TCG Ser TAG stop TGG Trp ССT Pro CCC Pro CCA Pro CCG Pro CGT Arg CGC Arg CGA Arg CGG Arg CTT Leu CAT His T CTC Leu CAC His CTA Leu CAA Gin A CTG Leu CAG GIn ATT lle АСT Thr AAT Asn AGT Ser ATC lle ACC Thr AAC Asn AGC Ser A ATA lle AAA Lys AAG Lys AGA Arg AGG Arg ACA Thr A ATG Met ACG Thr GAT Asp GAC Asp GGT Gly GGC Gly GGA Gly GTT Val GCT Ala GTC Val GCC Ala G GTA Val GCA Ala GAA Glu A GTG Val GCG Ala GAG Glu GGG Gly G third base in codon first base in codon
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