Tayer-relative amount of material spott *The TLC plates will be used are silica gel coa Different drugs will be used; Acetamina Salicylamide, and a mixture. * Procedure: * Dissolve the drugs in a mixture (1:1) of Ethan * The developing solvent is 0.5% glacial acetic * Spot diameter about 1-2 mm. Run the solver *The plates dimension about 10 x 6.6 cm. * Rf value (retardation value) = distance traveled by substance / distan *Recording: the Rf values of the used drugs an The Lab Report: briefly outline the procedure - of the unknown sample. 1) 6 Samples a, aspirin 3 6-salicylic acid 28213 drops 2) a. acetanilide 4 b. aniline 4.4 Just a dash of 1:1 Rf or Rf 3) a. dibromostilbine 4 b. stilbene 4.2 C CHROMATOGRAPHY "TLC" Chromatography is involved in the most modern and sophisticated methods of separating mixtures available to the organic chemist. Chromatography is defined as the separation of mixture of two or more different compounds or ions by distribution between two phases, one of which is stationary and the other of which is moving (mobile). Various types of chromatography are possible, depending on the nature of the two phases involved: A. Solid-liquid: a. Column chromatography (sizing and ion-exchange, affinity columns). b. Thin layer chromatography [TLC] c. Paper chromatography [PC] B. Liquid-liquid: High performance liquid chromatography [HPLC, FPLC] C. Gas-liquid (vapor-phase): Gas chromatography [GC] All chromatography works on much the same principle. Basically, the method depends on the differential solubilities or absorptivity of the substances to be separated relative to the two phases between which they are to be partitioned. In solid-liquid chromatograph the solids used most commonly are silica gel SiO2.xH20, alumina A1203.xH2O and sepharose. The possible interactions of organic compounds with alumina involve: coordination interaction, dipole-dipole interaction, or hydrogen bonding interaction. Solvent Selection is one of the parameters affecting the separation. Developing the TLC plates: UV light and lodine vapor in order to measure the Rf values. Identification of Drugs: TLC analysis of Analgesic Drugs * Factors affecting the separation in TLC: solvent system - adsorbent-thickness of the adsorbent layer - relative amount of material spotted. * The TLC plates will be used are silica gel coated with a fluorescent indicator. * Different drugs will be used; Acetaminophen, Aspirin, Caffeine, Ibuprofen, Acetanilide, Salicylamide, and a mixture. Procedure: * Dissolve the drugs in a mixture (1:1) of Ethanol: Methylene chloride. (One tablet per 10 mL. * The developing solvent is 0.5% glacial acetic acid in ethyl acetate. *Spot diameter about 1-2 mm. Run the solvent up to 0.5 cm from the top. * The plates dimension about 10 x 6.6 cm. * Rf value (retardation value) = distance traveled by substance / distance traveled by solvent front. * Recording: the Rf values of the used drugs and the unknown sample. The Lab Report: briefly outline the procedure-calculations-Revalues of each drug and the name of the unknown sample. 36