The glutamate dehydrogenase (GDH) catalyses the following reaction:H+H₂N- -C -COOCH₂CH₂COO™acide glutamique+ NAD + H₂OGDHTime (min)A340COO™c=oCH₂512341.760 1.718 1.675 1.635 1.595CH₂COO+ NH4+ NADH + H+acide a-cétoglutariqueThe activity of GDH is monitored in the sense of the formation of glutamate using thefollowing conditions:0.2 mL of 5 M ammonium sulphate2.4 mL of buffer at pH 80.1 mL of NADH at 6.15 mg.mL-¹ (M = 709 g.mol-¹)0.2 mL of 1 M a-ketoglutarate solutionWarm mixture at 25 °C for 5 minAdd 0.1 mL of GDH solution containing 1.6 mg.mL-¹protein to start thereaction.The change in absorbance at 340 nm is monitored, in a 1-cm cuvette, every minutefor 10 min. Results are given in the table below:Data ENADH at 340 nm = 6220 M¹.cm76789 101.550 1.510 1.489 1.476 1.451Calculate ammonium sulphate, NADH,concentrations in the reaction medium at t = 0.proteins- Draw the graph A = f(t). Calculate A340 at t = 0 and place this point on the curve.- Calculate the initial rate V₁ of the reaction in M.s¹.a-ketoglutarate and

In order to calculate the concentration of species in the reaction medium at time =0, we need to…

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Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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- Calculate ammonium sulphate, NADH, a-ketoglutarate and proteins concentrations in the reaction medium at t = 0. - Draw the graph A = f(t). Calculate A340 at t = 0 and place this point on the curve. - Calculate the initial rate V₂ of the reaction in M.s1.