You design a protein with an N-terminal signal sequence and a single transmembrane domain. You also decide to tag your protein with GFP. If you want the GFP to beoutside the cell, where should the GFP appear in the amino acid sequence?а.Between the signal sequence and the transmembrane domainb.At the C-terminal end of the protein (i.e., the last part of the protein to be translatedс.Between the transmembrane domain and the C-terminusd. The GFP can be anywhere in the amino acid sequence

Aequorea victoria green fluorescent protein structure. When exposed to light in the blue to…

Answered: You design a protein with an N-terminal…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Similar questions

Wilms tumor 1, or nephroblastoma, is caused by mutations in the WT1 gene, which encodes a transcription factor. You have identified a novel variant in WT1: Arg422Pro. You have control cells and cells that have been engineered to carry the homozygous WT1 p.Arg422Pro mutation. You want to assess effects of this mutation on a variety of endpoints. For each endpoint listed below, choose the one technique is best suited to answer the question. Choose from: array CGH, qRT-PCR, qPCR, RNA-seq, FISH, in situ hybridization, western blot, immunostaining, WT1 ChIP-seq, WT1 ChIP-PCR, ATAC-seq, 3C Endpoint Technique? WT1 protein amount (quantitative) Western blot WT1 protein binding to all enhancers, genome-wide Chip-seq WT1 mRNA amount (quantitative) WT1 protein subcellular localization Quantitative assessment of all mRNAs in these cells (genome-wide) RNAseq Chromatin interactions between a specific WT1 chromatin binding site (identified above)…
Explain karyopherin exportins? What is it? What is its function? Why is iş important for the nucleus?
76) Nuclear localization signal can be a. Simple sequence in which we have a stretch of acidic amino acid next to each of b. bipartite nuclear localization signal in which we have a stretch of acidic amino are separated by neutral amino acids. c. Simple sequence in which we have a stretch of basic amino acid next to each of d. bipartite nuclear localization signal in which we have a stretch of acidic amino acids then basic amino acids.
If there is a mutation in importin that prevents it from interacting with the NLS of cargo proteins, what affect, if any, would this have on the UPR (unfolded protein response). A. Ran-GEF would bind to the importin promoting cargo entry into the nucleus and transcription of UPR response genes. B. Gene regulatory protein would not be imported into the nucleus thus preventing transcription of UPR response genes C. MRNA would not be imported into the nucleus thus preventing transcription of UPR response genes D. Ran-GTP would not be imported to the nucleus thus preventing transcription of UPR response genes E. No effect, these are completely unrelated processes.
Define (whether post-transcriptional, post-translational, transcription) and describe the regulation of the Ferretin mRNA in the diagram below.
Which DNA strand will serve as the template strand during the transcription of the RNA-coding sequence?
In a-c below, will the residue on the right-hand side increase or decrease the pKa of the residue on the left-hand side? Explain.
ATP and GTP play a few roles during transcription and translation. What are two roles that ATP plays and one role that GTP plays? GO S x2 x2 i T: B I
Consider the mechanism of the enzyme RNase:  What would happen to the Km (i.e., would it increase, decrease, or stay the same) if the his12 was mutated to a lysine? Explain.  What would happen to the Kcat (i.e., would it increase, decrease, or stay the same) if the his12 was mutated to a valine? Explain.

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