Based on the structural properties of your protein above , how resistant (or sensitive) would your protein be to heat denaturation and why
Q: 3. Subtilisin (Mol. weight 27,600) is a protease that can catalyze hydrolysis of certain amino acid…
A: Enzymes are high molecular weight protein molecules that catalyse biochemical reactions. The…
Q: Which of the following isomers of D-glucose would be found in aqueous solution of D-glucose pH 7?…
A: Glucose is an aldohexose with 4 chiral carbons. So, the total number of possible stereoisomers of…
Q: 1.The diagram below shows an outline of the aminotransferase mechanism that skips the specific steps…
A: Vitamin B6 is a collective term for three biomolecules: pyridoxine, pyridoxal and…
Q: To the right is a schematic diagram of site in the Michaelis complex of a-chy- motrypsin and a…
A: Chymotrypsin is a protease that cleaves a peptide at the C-terminal of aromatic amino acids like…
Q: You would like to identify the protein subunits in a macromolecular complex. What kinds of…
A: Proteins are the building blocks of amino acids. The kind, amount, and sequence of amino acids that…
Q: 10) Explain how the Km and [VO] of a reaction between a fixed amount of catalase and a gradually…
A: An allosteric inhibitor doesn't competes with the substrate for binding at active site. It binds to…
Q: Draw the structures of two dipeptides: aspartic acid-glutamine and methionine-isoleucine with NH2…
A: Proteins are composed of amino acids and each amino acid are linked via peptide bond to form peptide…
Q: 1. Give TWO (2) examples of sterols responsible for the synthesis of Vitamin D. How these sterols…
A: Note : Hi ! Thank you for the question. We are authorized to answer one question at a time. Since…
Q: amino acid T
A: The Fischer projection is a two-dimensional representation of a three-dimensional organic molecule…
Q: 13. What value do N-linked saccharides serve for membrane surface proteins? Which amino acid…
A: N-linked saccharides, also known as N-linked glycans, play several crucial roles in the function of…
Q: 4. Amphiphilic Lipids. Detergents are small amphiphilic molecules that tend to form micelles in…
A: Ionic detergents contain anionic or cationic head group long with their hydrophobic tails being…
Q: 10. What are the steps involved in the ubiquitination of proteins? (select all that apply) a. E1…
A: Ubiquitination is a crucial biological process that regulates protein function in cells. It includes…
Q: Acid phosphatases are an important group of enzymes that can be detected in human blood serum.Under…
A: Acid phosphatases are the enzymes that are responsible to hydrolyze organic phosphates when the pH…
Q: 3CLpro is a cysteine protease, which are among the most common proteases in biochemistry, however…
A: The 3 dimensional conformation of a biomolecule is highly specific and vital to its function,…
Q: 12. What value do O-linked saccharides serve for membrane surface proteins? Which amino acid…
A: O-linked saccharides, also known as O-linked glycans, are carbohydrates that are attached to…
Q: 3. Below is an image of sucrose. OH HOT HO HO OH HO (a) Using sucrose as a substrate, draw a…
A: Carbohydrates in their Closed conformation can exist in the form of either of the two anomeric…
Q: Draw all possible products of a single hydrolysis being completed on the following polypeptide:…
A: Hydrolysis of protein peptide is the breaking down the peptide bond by addition of water molecule.
Q: Cofactors, coenzymes, and prosthetic groups datang une pomm
A: Enzyme function:Enzymes are the biological catalysts which speed up the reactions in organisms.These…
Q: 13.13) Write the names (using the three letter abbreviation method) of all of the tripeptides that…
A: Tripeptide is the macromolecule formed from three amino acids. Amino acids are joined by peptide…
Q: o 1. Protein tyrosine phosphatase enzymes are important regula- tory enzymes. Clinical trials show…
A: Michaelis Menton equationFor a one-substrate enzyme-catalyzed reaction, the Michaelis-Menton…
Q: 22. Which of the following processes results to the formation of D-glucuronic acid in biological…
A: Carbohydrates are the polyhydroxy aldehyde and ketone. Carbohydrates are classified as the…
Q: Lysosyme catalyzes the hydrolysis of the carbohydrate linkage in part of the bacterial cell wall…
A: Introduction Lysosyme is an enzyme that catalyzes the hydrolysis of the carbohydrate linkage in part…
Q: Cyt cb562 will form a tetramer in the presence of Zn+2 or in the absence of Zn+2, it will form a…
A: INTRODUCTION : Cyt cb562 - Cytochrome cb562 is a variant of an Escherichia coli four-helix bundle…
Q: The enzyme that cleaves off the sialic acid from degrading glycoproteins are directed to the…
A: Glycoproteins are proteins that contain carbohydrate molecules. These carbohydrates play a role in a…
Q: 2. Amino acid analysis of the a heptapeptide gave the following residues: Asp Glu Leu Lys Met Tyr…
A: The objective of the question is to determine the sequence of amino acids in a heptapeptide based on…
Q: determine the sequence of the mystery protein.
A: Given the amino and composition of the peptide as Asp Leu Lys Met Phe Tyr al When we treat with…
Q: Which polysaccharide is cleaved by beta glucosidases? A. Amylose B. Glycogen C. Inulin D. Cellobiose…
A: Polysaccharide is of two types homo and heteropolysaccharides. Homopolysaccharides have a single…
Q: Draw me the inhibition reaction mechanism of Cysteine Protease Inhibitor (E64). How the epoxide ring…
A: E64 is a cysteine protease inhibitor. Cysteine proteases are enzymes that have a cysteine residue at…
Q: 2) A. What is meant by energetic coupling? What is meant by the term phosphorylation and what role…
A: Answer: Introduction: Energy coupling means it takes place if the energy formed by one reaction or…
Q: Which of the following is true for the lipids with specific blological function Select an answer and…
A: Cell signaling is mediated by a ligand (usually a hormone but could be other molecules too) binding…
Q: Describe the tertiary level of protein structure and state the type of interactions present.
A: Since you have asked multiple questions, we will solve the first question for you. If you want any…
Q: IX. Insulin, a hormone vital in blood sugar regulation and having a polypeptide chain with disulfide…
A: Insulin is a hormone responsible for the regulation of sugar levels in the blood. Insulin hormone is…
Q: 10. Carboxypeptidase cleaves amino acids, one at a time, from the carboxyl end of the peptide chain.…
A: Enzymes are highly specialized proteins that have extraordinary catalytic power, greater than that…
Q: 2. Selective synthesis of the tetrapeptide Ala-Val-Gly-Leu a. Work backward from Structure K to fill…
A: Peptides can be synthesized chemically. For this we use various reagents like Boc (also called…
Q: 2. The following tyrosine-phosphorylated polypeptides were tested as substrates of protein tyrosine…
A: Enzymes are proteins that catalyze various reactions inside the cellular environment. Enzyme binds…
Q: 19. Define the terms “cofactor” and “coenzyme.” 20. What is the difference between general acid-base…
A: Enzymes are biocatalysts that speed up the reaction. Enzymes decrease the activation energy of a…
Q: Help filling in the blanks: Pyridoxal phosphate, PLP, is produced from pyridoxine or vitamin B6.…
A: Pyridoxal phosphate: It is the active form of vitamin B6. PLP is an important enzyme of amino acid…
Q: 11. This below notation represents a Bi-Bi reaction. Aspartate Oxaloacetate a-Ketoglutarate…
A: Introduction In ping pong reactions, the first substrate binds to the enzyme, transfers a piece of…
Q: 6. What is the catalytic triad and what particular protein family would you find this? What is the…
A: Serine proteases are protein cleaving enzymes that have Serine, Histidine and Aspartate (the…
Q: a. Briefly discuss Ramchandran plot. b. What is quaternary structure of proteins? Mention the role…
A: The Ramachandran plot:It is a plot of the torsional angles of the amino acid residues in a peptide…
Q: 5. Shown below are the first steps in the mechanism of a cysteine protease, which catalyses amide…
A: Cysteine proteases are known for their ability to cleave proteins in a highly specific manner and…
Q: Draw the products that would be produced from phospholipase C cleavage of a glycerophospholipid with…
A: Glycerophospholipids: They are fatty acid diglycerides with a phosphatidyl easter group attached to…
Q: 5. Refer to Model 2 for information relevant to this question. A sample of the peptide Lys net…
A: Amino acids are organic compound having two functional group namely carboxyl and amino group. Some…
Q: 1. Examine the synthetic glycoprotein, Molecule A, shown below: Protein- OH OH OH OH OH ÓH OH…
A: The polymer given in the question consists of glycoprotein molecule with the different sugar…
Q: Glycosphingolipids function via cis regulation and trans recognition. Explain these terms and…
A: Introduction: Glycosphingolipids (GSLs), are a subclass of glycolipids that consists of a…
Q: Given the following biosynthesis scheme, answer the questions (a)-(c) below. OH Me Li C E Me Me SACP…
A: For the synthesis of unbranched fatty acids, living beings carboxylate Acetyl CoA to yield malonyl…
5.
protein = D-Glucosaminate-6-phosphate Ammonia-lyase
Based on the structural properties of your protein above , how resistant (or sensitive) would your protein be to heat denaturation and why?
Step by step
Solved in 2 steps
- 1. Depict the structure features in the active site of cysteine protease 2. provide a step-wise reaction mechanism to show the hydrolysis of the peptide bond catalyzed by a cysteine proteasea. Suppose that you have the peptide Ala-Gly-Tyr-His-Leu and you treat it with FDNB and then 6M HCl. Draw the structures of all the products that you will have in solution (assume all reactions to go to completion).Small molecules are used as inhibitors of protein action - as drugs. They most often do this by blocking the active site within the protein. Potential drugs can be screened computationally to determine if they are strongly bound to the protein. Figure 1 shows a possible conformation of a candidate drug molecule, 4-bromo-2- carboxymethylamide-pyrrole (abbreviation: BCMAP) at the active site of a protein (abbreviation: PR). Figure 2 shows the full protein structure whilst figure 3 shows a known inhibitor of the protein at the site, overlayed with another calculated conformer of BCMAP. (a) Explain what types of interactions, both intermolecular and intramolecular, that a molecular mechanics forcefield must be able to describe in order to be able to accurately determine the geometry of BCMAP in the protein. Identify which interactions will be the most important to describe accurately. Figure 1.4-bromo-2-carboxymethylamide-pyrrole (BCMAP) (C, N, O, and Br atoms in yellow, blue, red, and…
- 7. Chalcone isomerase catalyzes the reaction illustrated below. A series of modification experiments were performed on this enzyme to identify which amino acid residues might be in the active site and which might promote the cyclization reaction. OH Ho. Но Explain the following results: A) Acetic anhydride treatment inhibited enzyme activity. B) Pyrocarbonate inhibited activity and was found to modify 7 residues. However, this enzyme only contains 6 His and modification of these did not affect activity. C) lodoacetate inhibited activity but this effect coult be decreased in the presence of substrate or product. D) Methane methanethiol sulfonate modified the enzyme but did not inhibit catalytic turnover. In addition, this modification was reversed in the presence of B-mercaptoethanol. keNyons Rengentplease help me understand asap Please draw by hand a tripeptide of Glu, Gln, and 1 amino acid of your choice (Not Glu or Gln again) at pH 7.0 and 9.0. The only requirement is that the amino acid should be 1 of the amino acids with ionizable R groups. Peptide should have appropriate charges on groups. Please1. Draw the Fisher projection of D-glucose, and from Fisher to Haworth projection of ẞ-D-glucose. Label (R) and (S) configuration for each chiral carbon Fisher projection Haworth projection
- 3. One strategy for designing an antiviral drug against hepatitis C virus (HCV) is targeting on the viral protein NS3-4A protease, an enzyme breaking the peptide chain through a nucleophilic acyl substitution reaction on amide. The designed drug Narlaprevir with a keto amide functional group acts as a protease inhibitor undergoing a nucleophilic addition reaction. 1) Propose a reasonable mechanism for protease (viral enzyme) breaking the viral protein Enz-OH (Viral protease) R Enz НА H2N R Viral protein O:Non-enzymatic browning Proteins: Lysin, methionine Sugars: glucose, galactose, sucrose, fructose, maltose 1. Describe (the aromas produced) and the degree of browning for each solution 2. Explain variations in color with respect to each sugar-amino acid combination by taking the chemical structures of sugars and amino acids into consideration1. In the mechanism of action of triose phosphate isomerase, Lys12 is an essential amino acid in the flexible phosphate gripper loop that undergoes a large conformational change to trap the substrate in the active site excluding water and holding the phosphate group in the correct geometry for isomerization rather than dephosphorylation. What would be the effect of the mutation of Lys12 to the unusual amino acid shown below? Explain. protein backbone
- 13. Amino acid analysis of a seven residue long peptide gave Asp Leu Arg Met | Phe Met | Phe | Tyr (i) The PTH amino acid derivative released by Edman degradation was Tyr-PTH (ii) Trypsin treatment had no effect (iii) Cyanogen bromide treatment yielded a dipeptide, a tetrapeptide, and free Arg (iv) Brief chymotrypsin treatment yielded several products, including a dipeptide and a tetrapeptide. The amino acid composition of the tetrapeptide was Leu, Arg, and Met What is the amino acid sequence of this seven residue long peptide, and explain how you reached that conclusion?I lea the - Oligopeptide 2 a. Give the three-letter and one-letter names of the amino acids in each oligopeptide starting from the amino terminal to the carboxyl terminal. Make sure that the amino acid names are arranged in CORRECT order for both oligopeptides. I b. After complete hydrolysis, which oligopeptide will yield more ATP molecules? How many NADH and FADH2 molecules will be theoretically produced? Show all relevant computations for each oligopeptide. Tabulate your answers to keep your answers organized. ( Oligopeptide Net ATP molecules Net NADH produced Net FADH2 produced produced Oligopeptide 1 Oligopeptide 2explain the correct and wrong choices
