Based on the data shown where is the DNA binding domain? Explain which constructs helped you reach this conclusion? Which part of the protein is the Activation domain? Explain which constructs helped you reach your conclusion? The schematic (a)below is that of a reporter-gene construct - containing a lacz reporter gene (encodingB-galactosidase) and a TATA box ligated to UASGAL, a regulatory element that contains several bindingsites for transcription factor Gal4.(a) Reporter-gene construct(b) Expression Vector construct: Various plasmids containing coding sequences of wild-type or mutant(portions of coding sequence deleted) Gal4 were made. (Plasmid 1 in diagram below)Gene encodingprotein XBoth the reported gene construct and plasmids with DNA encoding wild-type or mutant (deleted) Gal4were simultaneously introduced into mutant (gal4) yeast cells. (See schematic below)Plasmid12 activities were measured: In vitro binding (+ or -) to the reporter using EMSA's and the ability oftruncated proteins to activate transcription via production of reporter gene (B-gal activity) levels.X-bindingsiteNucleusDiReporterUAS GALgenePlasmidProtein XTATAboxReporter-genetranscripts(b) Wild-type and mutant Gal4 proteinsBindingto UAS GALWild typeDeletionconstructs1234lacz gene5501 741 74881692881755738881B-Galactosidaseactivity++++++

Name: Based on the data shown where is the DNA binding domain? Explain whic
Uploaded: 2024-03-20T06:42:41.000Z
Channel: Bartleby
Description: Based on the data shown where is the DNA binding domain? Explain which constructs helped you reach this conclusion? Which part of the protein is the Activation domain? Explain which constructs helped you reach your conclusion? The schematic (a)bel