As a graduate student, you join a lab and you are looking through some old notebooks. You find a linkage map someone has drawn. G----15 mu-- -T----10 mu-------B If you plan to complete a test cross with a GtB/gTb male, A) What are all of the expected progeny genotypes? B.) How many of each genotype would you expect if you collected 1000 progeny?
As a graduate student, you join a lab and you are looking through some old notebooks. You find a linkage map someone has drawn. G----15 mu-- -T----10 mu-------B If you plan to complete a test cross with a GtB/gTb male, A) What are all of the expected progeny genotypes? B.) How many of each genotype would you expect if you collected 1000 progeny?
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### Understanding Genetic Linkage Maps and Test Crosses
#### Introduction
As a graduate student, you join a lab and you are looking through some old notebooks. You find a linkage map someone has drawn:
```
G-------15 mu-------T-------10 mu-------B
```
#### Genetic Linkage Map Explanation
- **G, T, B**: These are genes.
- **15 mu, 10 mu**: These represent map units (mu), indicating the distance between the genes. One map unit usually correlates to a 1% recombination frequency between genes.
If you plan to complete a test cross with a GtB/gTb male:
A) **What are all of the expected progeny genotypes?**
B) **How many of each genotype would you expect if you collected 1000 progeny?**
#### Detailed Analysis
- **Parental Genotypes**: GtB and gTb.
- **Expected Progeny Genotypes**: Given the distances, you will get recombinants and non-recombinants. Use the map units to predict the frequencies:
1. **Non-recombinants** (GtB and gTb)
2. **Single Crossovers** (at two regions: G-T and T-B)
3. **Double Crossovers**
- **Expected Genotype Rates Based on Map Units**:
- **G-T**: 15% single crossover.
- **T-B**: 10% single crossover.
- **Double crossover**: Multiply the single crossover frequencies across two regions (0.15 * 0.10 = 0.015 or 1.5%).
#### Calculation for 1000 Progeny:
- Total crossovers in G-T = 15% -> 150 progeny
- Total crossovers in T-B = 10% -> 100 progeny
- Double crossovers = 1.5% -> 15 progeny
Split these crossovers equally and account for both single and double crossovers.
Parental/Non-recombinant Types:
- GtB (42.5%) -> 425
- gTb (42.5%) -> 425
Single Crossovers:
- Gtb (7.5%) -> 75
- gTB (7.5%) -> 75
Double Crossovers:
- gTB (1.5%) ->"
Transcribed Image Text:---
### Understanding Genetic Linkage Maps and Test Crosses
#### Introduction
As a graduate student, you join a lab and you are looking through some old notebooks. You find a linkage map someone has drawn:
```
G-------15 mu-------T-------10 mu-------B
```
#### Genetic Linkage Map Explanation
- **G, T, B**: These are genes.
- **15 mu, 10 mu**: These represent map units (mu), indicating the distance between the genes. One map unit usually correlates to a 1% recombination frequency between genes.
If you plan to complete a test cross with a GtB/gTb male:
A) **What are all of the expected progeny genotypes?**
B) **How many of each genotype would you expect if you collected 1000 progeny?**
#### Detailed Analysis
- **Parental Genotypes**: GtB and gTb.
- **Expected Progeny Genotypes**: Given the distances, you will get recombinants and non-recombinants. Use the map units to predict the frequencies:
1. **Non-recombinants** (GtB and gTb)
2. **Single Crossovers** (at two regions: G-T and T-B)
3. **Double Crossovers**
- **Expected Genotype Rates Based on Map Units**:
- **G-T**: 15% single crossover.
- **T-B**: 10% single crossover.
- **Double crossover**: Multiply the single crossover frequencies across two regions (0.15 * 0.10 = 0.015 or 1.5%).
#### Calculation for 1000 Progeny:
- Total crossovers in G-T = 15% -> 150 progeny
- Total crossovers in T-B = 10% -> 100 progeny
- Double crossovers = 1.5% -> 15 progeny
Split these crossovers equally and account for both single and double crossovers.
Parental/Non-recombinant Types:
- GtB (42.5%) -> 425
- gTb (42.5%) -> 425
Single Crossovers:
- Gtb (7.5%) -> 75
- gTB (7.5%) -> 75
Double Crossovers:
- gTB (1.5%) ->
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