A recessive maternal effect mutant in zebrafish, called ichabod, results in embryos lacking heads that are non-viable. You have been instructed to identify females that are homozygous for the ichabod mutant allele. At your disposal are a tank of wild-type fish (males and females), a tank of male and female parental fish that are all heterozygous for the ichabod mutant allele (ichabodl+), and a tank of F1 fish derived from a cross between a heterozygous male and heterozygous female (ichabodl+). Which of the following would be a way to identify females that are homozygous mutant, i.e. ichabodlichabod? Select all answers that would work. This experiment is not possible because homozygous females are not viable. O a. Cross F1 females to F1 males to make the F2 generation. Cross F2 females to F2 males and observe their offspring. Crosses that produce headless offspring came from a homozygous female. O b. Cross F1 females to F1 males and observe their offspring. Crosses that produce headless offspring came from a homozygous O c. female. Cross F1 males to females from the parental tank and observe their offspring. 25% of these crosses should produce headless offspring. Od. Cross F1 females to wild-type males and observe their offspring. Crosses that produce headless offspring came from a homozygous female. O e.
A recessive maternal effect mutant in zebrafish, called ichabod, results in embryos lacking heads that are non-viable. You have been instructed to identify females that are homozygous for the ichabod mutant allele. At your disposal are a tank of wild-type fish (males and females), a tank of male and female parental fish that are all heterozygous for the ichabod mutant allele (ichabod/+), and a tank of F1 fish derived from a cross between a heterozygous male and heterozygous female (ichabod/+). Which of the following would be a way to identify females that are homozygous mutant, i.e. ichabod/ichabod? Select all answers that would work.

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Maternal effect in zebrafish is a situation where the phenotype of an organism is determined not only by the environment it experiences and its genotype, but also by the environment and genotype of its mother.
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