Agarose gel clectrophoresis is a technique used to separate DNA fragments on an agarosematrix. The DNA is then viewed by staining or under UV light.1068(i)What is the direction of DNA migration in electric field on the agarose gel?(ii)Explain the principle for the migration of DNA molecules in an electric field.(iii) DNA loading dye is used to prepare DNA markers and samples for loading on theagarose gel. State the function of bromophenol blue in the loading dye.

In biotechnology the separation of samples like DNA, RNA and proteins are performed by gel…

Answered: Agarose gel clectrophoresis is a…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Compare the melting temperature of a 1-kb segment of DNA containing20% A residues to that of a 1-kb segment containing 30% A residues under the same conditions.
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Why is DNA not soluble in ethanol whereas other components cells are soluble. What is the rationale behind DNA precipitation in solution
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Question 12 Referring to the pBR322 plasmid below, if a recombinant plasmid were obtained inserting DNA into the BamHI site, the recombinant plasmid would lack which of the following properties? A It would replicate autonomously. B) It would be resistant to the antibiotic ampicillin. C) It would possess at least two promoter sites. (D) It would be resistant to the antibiotic tetracycline. Pul Pod- ampr HIID EcoRI BamHI pBR322 (4363 bases) or Prull let Aval -Sall
a.) Explain briefly how silica allows us to purify DNA. Be sure to state the relevant biochemical features of silica and DNA as well as any relevant reagents or steps that are important for the purification. Your answer should explain both how we get the DNA to bind in the first place, and how we are able to remove the DNA. You do not (and should not) explain earlier steps in a DNA purification; focus you answer on the silica:DNA interaction. b.) In theory, can a silica spin column also be used to purify RNA? If so, explain how (and why we did not observe RNA when analyzing plasmid DNA via agarose gel electrophoresis). If not, explain why not.
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Question 8. You isolate the DNA from the bacterial cells and apply the Sanger dideoxy sequencing method. You then separate the products of the reactions by gel electrophoresis and obtain the following pattern. What is the sequence of the template and the DNA on the gel? ddATP ddTTP ddCTP ddGTP Template V [ Choose 3'-CTAGTCAAGG-5' 5'-CTAGTCAAGG-3' Sequence 3'-GATCAGTTCC-5' 5'-GATCAGTTCC-3' 5'-GGAACTGATC-3'
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A plot showing the % of denautration as a function of temperature for a melting point of a DNA sample under 0.12 M NaCl gives an equation of a line of Y= 0.0074 X - 0.044 (Where Y is the % of denautration and X is the temperature in degree C) This is all the information that was given. 1. Calcuate the melting point of this DNA in degree C
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