Agarose gel clectrophoresis is a technique used to separate DNA fragments on an agarose matrix. The DNA is then viewed by staining or under UV light.1068 (i) What is the direction of DNA migration in electric field on the agarose gel? (ii) Explain the principle for the migration of DNA molecules in an electric field. (iii) DNA loading dye is used to prepare DNA markers and samples for loading on the agarose gel. State the function of bromophenol blue in the loading dye.
Agarose gel clectrophoresis is a technique used to separate DNA fragments on an agarose matrix. The DNA is then viewed by staining or under UV light.1068 (i) What is the direction of DNA migration in electric field on the agarose gel? (ii) Explain the principle for the migration of DNA molecules in an electric field. (iii) DNA loading dye is used to prepare DNA markers and samples for loading on the agarose gel. State the function of bromophenol blue in the loading dye.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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![Agarose gel clectrophoresis is a technique used to separate DNA fragments on an agarose
matrix. The DNA is then viewed by staining or under UV light.1068
(i)
What is the direction of DNA migration in electric field on the agarose gel?
(ii)
Explain the principle for the migration of DNA molecules in an electric field.
(iii) DNA loading dye is used to prepare DNA markers and samples for loading on the
agarose gel. State the function of bromophenol blue in the loading dye.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fcd502576-ec95-4f2f-a8d3-2a0ce2b71ac3%2F82a6ba61-43b8-4b3c-873f-30d4033052e9%2Fmedtvgou_processed.jpeg&w=3840&q=75)
Transcribed Image Text:Agarose gel clectrophoresis is a technique used to separate DNA fragments on an agarose
matrix. The DNA is then viewed by staining or under UV light.1068
(i)
What is the direction of DNA migration in electric field on the agarose gel?
(ii)
Explain the principle for the migration of DNA molecules in an electric field.
(iii) DNA loading dye is used to prepare DNA markers and samples for loading on the
agarose gel. State the function of bromophenol blue in the loading dye.
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