In one reaction used for DNA sequencing 2,3 dideoxy-cytosine tri-phosphate is added to the reaction mixture. Why? It is used to fluorescently mark cytosine in the DNA chain. O O O O O It is used as a primer to start synthesis at positions along the DNA that complement cytosine. It is used to terminate DNA chain growth at sites where cytosine occurs. It is used to stimulate the polymerase chain reaction and allow the template to be copied It is used to bind to and to separate the DNA pieces on the gel.

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Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.