a) Which of the following is not required for PCR? dNTPs bacterial plasmids carefully designed primers taq or other heat-resistant polymerases template DNA b) Sanger sequencing (aka the chain termination method) is a method for determining the sequence of nucleotides in a piece of DNA of interest requires use of special ddNTPs (dideoxy nucleotides) that have special fluorescent labels (dyes) attached to them and also lack a 3' hydroxyl requires fragments to be run through a tube containing a gel matrix where their fluorescent dyes are illuminated with a laser, which can be used to produce a chromatogram all of the above are true none of the above are true c) The technique that allows quick and efficient test-tube replication of DNA is: PCR Restriction enzyme analysis Dideoxy sequencing DNA gel electrophoresis Gene therapy
Genetic Recombination
Recombination is crucial to this process because it allows genes to be reassorted into diverse combinations. Genetic recombination is the process of combining genetic components from two different origins into a single unit. In prokaryotes, genetic recombination takes place by the unilateral transfer of deoxyribonucleic acid. It includes transduction, transformation, and conjugation. The genetic exchange occurring between homologous deoxyribonucleic acid sequences (DNA) from two different sources is termed general recombination. For this to happen, an identical sequence of the two recombining molecules is required. The process of genetic exchange which occurs in eukaryotes during sexual reproduction such as meiosis is an example of this type of genetic recombination.
Microbial Genetics
Genes are the functional units of heredity. They transfer characteristic information from parents to the offspring.
a) Which of the following is not required for PCR?
- dNTPs
- bacterial plasmids
- carefully designed primers
- taq or other heat-resistant polymerases
- template DNA
b) Sanger sequencing (aka the chain termination method)
- is a method for determining the sequence of nucleotides in a piece of DNA of interest
- requires use of special ddNTPs (dideoxy nucleotides) that have special fluorescent labels (dyes) attached to them and also lack a 3' hydroxyl
- requires fragments to be run through a tube containing a gel matrix where their fluorescent dyes are illuminated with a laser, which can be used to produce a chromatogram
- all of the above are true
- none of the above are true
c) The technique that allows quick and efficient test-tube replication of DNA is:
- PCR
- Restriction enzyme analysis
- Dideoxy sequencing
- DNA gel electrophoresis
- Gene therapy
Step by step
Solved in 2 steps
