DNA Fingerprinting Experiment Daughter Mother Possible Fathers Horse Horse 1 2 3 4 The second and third bands down in the mother horse are not present in the daughter horse. What would be the most likely reason? The daughter horse suffered from a serious mutation that eliminated them from her genome. The bands were too small to be seen in the daughter horse. The daughter horse did not inherit the chromosome from the mother horse that those bands came from. The daughter horse inherited them from the father horse.

Anspart a, b and c with clear wordings

Take a look at question 3 in More Genetic TIPS. Let’s suppose amale is heterozygous for two polymorphic sequence-tagged sites.STS-1 exists in two sizes: 211 bp and 289 bp. STS-2 also exists intwo sizes: 115 bp and 422 bp. A sample of sperm was collectedfrom this man, and individual sperm were placed into 30 separatetubes. Into each of the 30 tubes were added the primers that amplifySTS-1 and STS-2, and then the samples were subjected toPCR. The following results were obtained:

Are you a hidden heterozygote? A PCR analysis (part2) Agarose gel electrophoresis and interpretation la: Several factors (including agarose gel concentration, time and current) affect migration of DNA fragments through the agarose gel. Briefly explain how each of these factors affects DNA migration. Agarose gel concentration: Time: Voltage: 1b: Do DNA fragments move towards the positive or negative end of the gel box? Explain your answer. 1c: What is the purpose of the Tris-Acetate-EDTA (TAE) buffer that the agarose gel is prepared with and submerged in for running? What would happen if you used water to prepare and run the gel instead of TAE buffer? 1d: If the student is homozygous for the brown allele, how many bands will they see in the lanes for the blue and brown allele samples? (circle one) Brown sample: 0 Blue sample: 1 2 more than two. 1 2 more than two. le: If the student is homozygous for the blue allele, how many bands will they see in the lanes for the blue and brown allele…