A region of the genome is amplified by PCR to analyze two closely linked SNPs. PCR products from individual A were labelled with a red-fluorescing dye and those from individual B labelled with a green-fluorescing dye. These PCR products were mixed and hybridized to an oligonucleotide microarray as shown in the figure below. What can we conclude about the data shown?

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A region of the genome is amplified by PCR to analyze two closely linked SNPs. PCR products from individual A were labelled with a red-fluorescing dye and those from individual B labelled with a green-fluorescing dye. These PCR products were mixed and hybridized to an oligonucleotide microarray as shown in the figure below. What can we conclude about the data shown?

Select all true:

a. individual A is heterozygous for the M1 and M2 haplotypes, individual B is heterozygous for M4 and M5

b. individual A is heterozygous for the M1 and M2 haplotypes, individual B is heterozygous for M3 and M6

c. Sanger sequencing traces of these PCR products would show the same double peaks at the same positions for these two individuals

d. analyzing this PCR amplified region with conventional Sanger sequencing would be more accurate than the microarray analysis

e. individuals A and B could be siblings who share a parent that is homozygous for one of the haplotypes

 

{urgent} 

M1
M2
M3
M4
M5
M6
The oligonucleotides on the array are:
M1: 5' ACTTACCGAGAGAACCTGCG 3'
M2: 5' ACTGACCGAGAGAGCCTGCG 3'
M3: 5' ACTTACCGAGAGAGCCTGCG 3'
M4: 5' ACTCACCGAGAGACCCTGCG 3'
M5: 5' ACTCACCGAGAGATCCTGCG 3'
M6: 5' ACTGACCGAGAGAACCTGCG 3'
Transcribed Image Text:M1 M2 M3 M4 M5 M6 The oligonucleotides on the array are: M1: 5' ACTTACCGAGAGAACCTGCG 3' M2: 5' ACTGACCGAGAGAGCCTGCG 3' M3: 5' ACTTACCGAGAGAGCCTGCG 3' M4: 5' ACTCACCGAGAGACCCTGCG 3' M5: 5' ACTCACCGAGAGATCCTGCG 3' M6: 5' ACTGACCGAGAGAACCTGCG 3'
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