A protein X is extensively methylated at lysine residues, converting it to protein Y. If the protein is to be separated by cation exchanger what would be the order of elution? A.X, followed by Y OB. Y, followed by X OC. Both will be eluted at the same time D. Both will bind with the matrix in the exchanger and will not elute out E. A cation exchanger is not the ideal matrix for the separation
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- Ion transporters are “linked” together—not physi-cally, but as a consequence of their actions. For example,cells can raise their intracellular pH, when it becomes tooacidic, by exchanging external Na+ for internal H+, usinga Na+–H+ antiporter. The change in internal Na+ is thenredressed using the Na+-K+ pump.A. Can these two transporters, operating together,normalize both the H+ and the Na+ concentrations insidethe cell?B. Does the linked action of these two pumps causeimbalances in either the K+ concentration or the mem-brane potential? Why or why not?The figure below shows amino acid interactions between the a /B2 subunit interface of hemoglobin. These interactions stabilize the T state of the protein. Based on the figure below, which amino acid in the picture below, if replaced with glutamate, would decrease the Ka of hemoglobin for O2 the most? Include both the residue and the residue number in your response (For example, V1 or K127). to B, V34 8- a, R141 D126 K127 NoncovalentTwo proteins, A and B, bind to the same ligand, L, with the binding curvesshown below. What is the dissociation constant, Kd , for each protein? Which protein (A or B) has a greater affinity for ligand L?
- What can be deduced from the following information? N- C- Protein Nonpolar polar acidic basic term term 33 42 10 15 Ser Asp Y 33 42 10 15 Ser Asp A. The two proteins, X and Y are one and the same B. The two proteins are globular proteins that are hydrophilic C. The two proteins are most likely localized in the cytoplasm D. X and Y most likely have transport function for various types of metabolites E. The data is inconclusive and similarity or differences between X and Y cannot be determinedDefine the following terms:a. cotranslational transferb. posttranslational transferc. TOM receptor proteind. TIM complexe. mthsp70Transport of histidine into a bacterial cell was measured at several different histidine concentrations, see table below. Based on these data, is histidine uptake facilitated by a carrier or channel protein? a b Histidine (uM) 2.5 7 16 31 72 с Select an answer and submit. For keyboard navigation, use the up/down arrow keys to select an answer. A carrier protein because it becomes saturated. A carrier protein because it does not become saturated. A channel protein because it becomes saturated. Transport (uM/min) d A channel protein because it does not become saturated. 42.5 119 240 490 1000 X Your answer
- If you were to remove the ER retrieval signal (KDEL) from protein disulfide isomerase (PDI), which is normally a soluble resident of the ER lumen, where would you expect the modified PDI to be located? Why?Protein A has a binding site for ligand X with a Kd of 10 −6 M. Protein B has a binding site for ligand X with a Kd of 10 −9 M. Which protein has a higher affinity for ligand X? Explain your reasoning. Convert the Kd to Ka for both proteins.A peptide with the sequence AELQAKSAIAHELQAKAAIAHA is treated with ATP while in the presence of kinase An alpha helix is formed with the phosphorylated at pH 5 In the direction of the helix axis, what is the length of the helix
- Below is an image of a calcium binding site on the calmodulin protein. What statement is correct regarding this Ca2+ binding? 140 ASN 136 GL IL 29 131 GL 132 133 Time left 1:1 O a. deprotonated acidic side chains are important for Ca2+ binding O b. Ca2+ binding usually does not result in conformational changes in a protein O c. deprotonated basic residues are important for Ca2+ binding O d. Ca2+ binding to proteins is usually done by coordination with one side chainA transmembrane protein helps catalyze the transport of solute A from the INSIDE TO the OUTSIDE of a cell. The reaction can be represented by the reaction coordinate diagram below. It is possible to conclude that the concentration of A on the outside of the cell is than its concentration on the inside AND that it is_ to expect that the protein will at some point form a continuous path between th cytosol and the extracellular environment. A inside A outside reaction coordinate O lower; reasonable O higher; unreasonable O higher; reasonable free energyDescribe the effect on the protein if the base pair #6 in the parent DNA chain is misted from G/C to A/T.