A protein X is extensively methylated at lysine residues, converting it to protein Y. If the protein is to be separated by cation exchanger what would be the order of elution? A.X, followed by Y OB. Y, followed by X OC. Both will be eluted at the same time D. Both will bind with the matrix in the exchanger and will not elute out E. A cation exchanger is not the ideal matrix for the separation

Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter20: Electron Transport And Oxidative Phosphorylation
Section: Chapter Questions
Problem 22P
icon
Related questions
Question
A protein X is extensively methylated at lysine residues, converting
it to protein Y. If the protein is to be separated by cation exchanger,
what would be the order of elution?
CAX, followed by Y
OB. Y, followed by X
OC. Both will be eluted at the same time
O D. Both will bind with the matrix in the exchanger and will not elute out
OE. A cation exchanger is not the ideal matrix for the separation
Transcribed Image Text:A protein X is extensively methylated at lysine residues, converting it to protein Y. If the protein is to be separated by cation exchanger, what would be the order of elution? CAX, followed by Y OB. Y, followed by X OC. Both will be eluted at the same time O D. Both will bind with the matrix in the exchanger and will not elute out OE. A cation exchanger is not the ideal matrix for the separation
Expert Solution
steps

Step by step

Solved in 2 steps

Blurred answer
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning