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A protein was recently discovered to be located in the nucleus. However, it is uncertain whether this is a protein that permanently stays in the nucleus or a shuttling protein that stays most of the time in the nucleus. One way to resolve this question is to perform the following steps: • Fuse the cells expressing the fluorescently labeled protein in their nucleus with the cells that are not expressing it. • Treat the cells with an inhibitor of protein synthesis to prevent the synthesis of more labeled proteins. • Observe for fluorescence in the fused cell with two nuclei. c. Why is it important to stop the synthesis of more of the labeled proteins?

A protein was recently discovered to be located in the nucleus. However, it is uncertain whether this is a protein that permanently stays in the nucleus or a shuttling protein that stays most of the time in the nucleus. One way to resolve this question is to perform the following steps: • Fuse the cells expressing the fluorescently labeled protein in their nucleus with the cells that are not expressing it. • Treat the cells with an inhibitor of protein synthesis to prevent the synthesis of more labeled proteins. • Observe for fluorescence in the fused cell with two nuclei. c. Why is it important to stop the synthesis of more of the labeled proteins?

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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ANSWER QUESTION C and EXPLAIN A protein was recently discovered to be located in the nucleus. However, it is uncertain whether this is a protein that permanently stays in the nucleus or a shuttling protein that stays most of the time in the nucleus. One way to resolve this question is to perform the following steps: • Fuse the cells expressing the fluorescently labeled protein in their nucleus with the cells that are not expressing it. • Treat the cells with an inhibitor of protein synthesis to prevent the synthesis of more labeled proteins. • Observe for fluorescence in the fused cell with two nuclei. c. Why is it important to stop the synthesis of more of the labeled proteins?
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