A fusion protein can be synthesized to carry a specific tag, which is useful in identifying the proteins in various experiments. Fusion proteins with the FLAG epitope bind to specific anti-FLAG antibodies and carry this extra sequence: Asp-Tyr-Lys-Asp-Asp- Asp-Asp-Lys. a. Draw the structure of the FLAG epitope at pH 7.0. b. As you have drawn it, what is the approximate net charge of the FLAG epitope at pH 7.0? c. How would the structure and net charge of the FLAG epitope change if placed into a pH 12.5 solution?

A fusion protein can be synthesized to carry a specific tag, which is useful in identifying the proteins in various experiments. Fusion proteins with the FLAG epitope bind to specific anti-FLAG antibodies and carry this extra sequence: Asp-Tyr-Lys-Asp-Asp- Asp-Asp-Lys. a. Draw the structure of the FLAG epitope at pH 7.0. b. As you have drawn it, what is the approximate net charge of the FLAG epitope at pH 7.0? c. How would the structure and net charge of the FLAG epitope change if placed into a pH 12.5 solution?

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter17: Genes And The Immune System

Section: Chapter Questions

Problem 13QP: The Adaptive Immune Response Is a Specific Defense Against Infection In cystic fibrosis gene...

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