A chain NH3 NH3 B chain Gly Phe 2. The protein pictured below is bovine insulin. Determine the number and the size of the fragments that would be generated upon treatment with the following: lle Val Val Aşn Gln Ġln 5 Gln 5 His look for the cleavage points (a) without DTT and (b) with DTT. Cys Leu Cys Cys Without DTT With DTT Ála Gly Ser Ser Trypsin 10 Val 10 His Cys Leu Chymotrypsin Val Ser Leu Glu BrCN Tyr Ala 15 Gln 15 Leu Leu Тyr Glu Leu Asn Val Tyr Cys 20 Čys- S' 20 Gly Asn Glu Arg Reagent (source) Trypsin (bovine pancrease) Chymotrypsin (bovine pancrease) Staphylococcus V8 protease Pepsin (porcine pancrease) Cyanogen bromide (chemical)(CnBr) Specificity Lys, Arg (C) Phe, Trp, Tyr (C) Glu, Asp (C) Phe, Trp, Tyr (N) Met (C) Gly Phe 25 Phe Tyr Thr Pro Lys 30 Åla

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
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A chain
NH3
NH3
B chain
Gly
Phe
2. The protein pictured below is bovine insulin. Determine the number
and the size of the fragments that would be generated upon treatment
with the following:
İle
Val
Val
Asn
Gln
Gln
5 Ġln
5 His
look for the cleavage points (a) without DTT and (b) with DTT.
Cys
Leu
Cys
S-S
Cys
Without DTT
With DTT
Ala
Ģly
Ser
Ser
Trypsin
10 Val
10 His
Cys
Leu
Chymotrypsin
Ser
Val
Leu
Glu
BrCN
Tyr
Ala
15 Gln
15 Leu
Leu
Тyr
Ġlu
Leu
Ásn
Val
Тyr
Cys
20 Çys
20 Gly
Asn
Glu
Arg
Reagent (source)
Trypsin (bovine pancrease)
Chymotrypsin (bovine pancrease)
Staphylococcus V8 protease
Pepsin (porcine pancrease)
Cyanogen bromide (chemical)(CnBr)
Specificity
Lys, Arg (C)
Phe, Trp, Tyr (C)
Glu, Asp (C)
Phe, Trp, Tyr (N)
Met (C)
Gly
Phe
25 Phe
Тyr
Thr
Pro
Lys
30 Ála
Transcribed Image Text:A chain NH3 NH3 B chain Gly Phe 2. The protein pictured below is bovine insulin. Determine the number and the size of the fragments that would be generated upon treatment with the following: İle Val Val Asn Gln Gln 5 Ġln 5 His look for the cleavage points (a) without DTT and (b) with DTT. Cys Leu Cys S-S Cys Without DTT With DTT Ala Ģly Ser Ser Trypsin 10 Val 10 His Cys Leu Chymotrypsin Ser Val Leu Glu BrCN Tyr Ala 15 Gln 15 Leu Leu Тyr Ġlu Leu Ásn Val Тyr Cys 20 Çys 20 Gly Asn Glu Arg Reagent (source) Trypsin (bovine pancrease) Chymotrypsin (bovine pancrease) Staphylococcus V8 protease Pepsin (porcine pancrease) Cyanogen bromide (chemical)(CnBr) Specificity Lys, Arg (C) Phe, Trp, Tyr (C) Glu, Asp (C) Phe, Trp, Tyr (N) Met (C) Gly Phe 25 Phe Тyr Thr Pro Lys 30 Ála
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