-. Succinate dehydrogenase contains iron-sulfur centers in which irons are complexed with cysteine residues. You mutate those cysteines to serines, which is a conservative mutation so it will not mess up the structure, but iron is not complexed effectively. a) What effect will this have on electron transport? Which complex will be affected? yes, complex 3, Complex 4, b) How many protons will be pumped per glucose molecule now assuming everything else continues as normal? How many total ATPs will you make per glucose for the entire glucose catabolism (again assuming everything else, including citric acid cycle continues as normal)? Only 4 protons pumped from #1 and 2H+ from #4 Glycolysis=2 ATP CAC = 2 ATP ETC=Ø 4 ATP c) Would everything actually continue as normal? What do you think would actually happen in cells? How many total ATPs would you make per glucose? 4 ATP, No, very low energy output slow growth + replication d) If the FADH₂ is subsequently oxidized by a matrix NAD* to regenerate it, how many ATP molecules will you now make from 1 glucose?

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
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Chapter1: Biochemistry: An Evolving Science
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Please check my work and make corrections for parts A, B, and C and explain the answer for part D. Thank you!
been removed by Kinase anyway. They only thing that
would change is not needing Kinase in that step.
4. Succinate dehydrogenase contains iron-sulfur centers in which irons are complexed with cysteine
residues. You mutate those cysteines to serines, which is a conservative mutation so it will not mess
up the structure, but iron is not complexed effectively.
a) What effect will this have on electron transport? Which complex will be affected?
Yes, complex 3, Complex 4,
b) How many protons will be pumped per glucose molecule now assuming everything else
continues as normal? How many total ATPs will you make per glucose for the entire glucose
catabolism (again assuming everything else, including citric acid cycle continues as normal)?
Only 4 protons pumped from #1 and 2H+ from #4
4 ATP
Glycolysis=2 ATP CAC = 2 ATP ETC-0
c) Would everything actually continue as normal? What do you think would actually happen in
cells? How many total ATPs would you make per glucose?
4 ATP, No, very low energy Output
slow growth + replication
d) If the FADH₂ is subsequently oxidized by a matrix NAD* to regenerate it, how many ATP
molecules will you now make from 1 glucose?
Transcribed Image Text:been removed by Kinase anyway. They only thing that would change is not needing Kinase in that step. 4. Succinate dehydrogenase contains iron-sulfur centers in which irons are complexed with cysteine residues. You mutate those cysteines to serines, which is a conservative mutation so it will not mess up the structure, but iron is not complexed effectively. a) What effect will this have on electron transport? Which complex will be affected? Yes, complex 3, Complex 4, b) How many protons will be pumped per glucose molecule now assuming everything else continues as normal? How many total ATPs will you make per glucose for the entire glucose catabolism (again assuming everything else, including citric acid cycle continues as normal)? Only 4 protons pumped from #1 and 2H+ from #4 4 ATP Glycolysis=2 ATP CAC = 2 ATP ETC-0 c) Would everything actually continue as normal? What do you think would actually happen in cells? How many total ATPs would you make per glucose? 4 ATP, No, very low energy Output slow growth + replication d) If the FADH₂ is subsequently oxidized by a matrix NAD* to regenerate it, how many ATP molecules will you now make from 1 glucose?
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