-. Succinate dehydrogenase contains iron-sulfur centers in which irons are complexed with cysteine residues. You mutate those cysteines to serines, which is a conservative mutation so it will not mess up the structure, but iron is not complexed effectively. a) What effect will this have on electron transport? Which complex will be affected? yes, complex 3, Complex 4, b) How many protons will be pumped per glucose molecule now assuming everything else continues as normal? How many total ATPs will you make per glucose for the entire glucose catabolism (again assuming everything else, including citric acid cycle continues as normal)? Only 4 protons pumped from #1 and 2H+ from #4 Glycolysis=2 ATP CAC = 2 ATP ETC=Ø 4 ATP c) Would everything actually continue as normal? What do you think would actually happen in cells? How many total ATPs would you make per glucose? 4 ATP, No, very low energy output slow growth + replication d) If the FADH₂ is subsequently oxidized by a matrix NAD* to regenerate it, how many ATP molecules will you now make from 1 glucose?

-. Succinate dehydrogenase contains iron-sulfur centers in which irons are complexed with cysteine residues. You mutate those cysteines to serines, which is a conservative mutation so it will not mess up the structure, but iron is not complexed effectively. a) What effect will this have on electron transport? Which complex will be affected? yes, complex 3, Complex 4, b) How many protons will be pumped per glucose molecule now assuming everything else continues as normal? How many total ATPs will you make per glucose for the entire glucose catabolism (again assuming everything else, including citric acid cycle continues as normal)? Only 4 protons pumped from #1 and 2H+ from #4 Glycolysis=2 ATP CAC = 2 ATP ETC=Ø 4 ATP c) Would everything actually continue as normal? What do you think would actually happen in cells? How many total ATPs would you make per glucose? 4 ATP, No, very low energy output slow growth + replication d) If the FADH₂ is subsequently oxidized by a matrix NAD* to regenerate it, how many ATP molecules will you now make from 1 glucose?

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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