A bacterium was isolated from the Pacific Ocean. Jenna is a rescarcher interested to study itsamylase gene. Before the gene could be further investigated, the genome of this bacteriumshould first be extracted.(i)Jenna plans to use enzymes, sonication, manual grinding and liquid homogenizationto achieve this objective. Elaborate in detailt these lysis methods.(ii)After the genome is obtained, explain how the anylase gene could be cloned intoEscherichia coli competent cells to be amplified.

Bacterial cell lysis is a method in which the cell membrane is broken down or destroyed in order to…

Answered: A bacterium was isolated from the…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Similar questions

Select all that apply from the following to each transformation process : The following are White non-glowing colonies White glowing colonies Blue non-glowing colonies Blue glowing colonies None of the above The transformation are: a. LB + kanamycin plates that have been spread with the E. coli cells transformed with your ligations, what phenotype of the colonies do you expect to obtain . b. LB + kanamycin plates that have been spread with the E. coli cells transformed with water, what phenotype of the colonies do you expect to obtain. c. LB plates that have been spread with the E. coli cells transformed with your pHSG298, what phenotype of the colonies do you expect to obtain. d. LB + kanamycin plates that have been spread with the E. coli cells transformed with PHSG298, what phenotype of the colonies do you expect to obtain. e. LB plates that have been spread with the E. coli cells transformed with your ligations, what phenotype of the colonies do you expect to obtain. f. LB plates…
Some bacteria may have higher mutation rates than others following exposure to UV light. Discuss a reason why this might be the case. What experiments could you do to determine whether this is a likely possibility?
You have sequenced the genome of the bacteriumSalmonella typhimurium, and you are using BLAST analysis to identify similarities within the S. typhimuriumgenome to known proteins. You find a protein thatis 100 percent identical in the bacterium Escherichiacoli. When you compare nucleotide sequences of theS. typhimurium and E. coli genes, you find that their nucleotide sequences are only 87 percent identical.a. Explain this observation.b. What do these observations tell you about the meritsof nucleotide- versus protein-similarity searches inidentifying related genes?
When present on the leaves of plants, the bacterium Pseudomonas syringae can promote frost damage to plants. Mutant strains, lacking the “ice” gene, have been applied to plants to try and protect the plants from wild-type P. syringae-induced frost. Assume that I am telling the truth that wild-type P. syringae nucleates ice formation at -2 °C, and provide an explanation as to why application of this altered (mutant) bacterium to plants might be a beneficial agricultural strategy in areas where morning lows occasionally dip down to 28-30 °F.
Describe the growth pathway the bacteriophage lambda will take when used to infect a mid-log-phase culture of E.coli
A paper in the Journal of Bacteriology reported that a mutT mutant strain of E. coli displayed a mutator phenotype when the bacteria were cultured anaerobically. Is this a surprising result? Briefly explain your answer. Present one or two possible explanations for this observation.
Please answer the following questions using numbers and not text. For example, if you think the answer is 24, write 24 and not twenty-four. If you think the answer is 24 ATP, write only the number 24. As a microbiology technician, your job is to characterize a novel bacterial strain isolated from insects. Phenotypically, you have discovered that the new bacterial strain can grow in the presence or absence of oxygen. After genome sequencing and biochemical characterization, you know that your new organism is capable of making all of the enzymes necessary for glycolysis and the TCA cycle, and that it has an electron transport chain. Answer the following questions based on the growth of your organism on minimal media plates containing only acetate as a carbon and energy source. Acetate is transported across the membrane at the cost of 3 ATP per molecule. Each question should be answered based on the metabolism of one molecule of acetate. If you come up with a negative value, indicate an…
From an Escherichia coli strain, five Hfr strains were isolated. The location and orientation of the transfer origin of each Hfr strain is shown in Figure 1. You want to use these five strains to map the locus responsible for thiamine synthesis, called thi. Each Hfr strain is sensitive to rifampicin (Rifs) and thi*. Conjugation experiments are performed between each of the Hfr strains and an F strain Rif Thi™. 0 T leu 10 20 T nadD pyrC trp 40 T his 60 70 2) The results are shown in the following table: Donor strain Hfr1 Hfr2 Hfr3 Hfr4 Hfr5 cysG 80 90 1) What is the selection medium used in these conjugation experiments metA Colonies Thi+ 1000 0 400 0 25 100 Hfr1 Hfr2 Figure 1: Chromosome map of Escherichia coli. Five Hfr strains (Hfr1 to Hfr5) were isolated and the location and orientation of the origin of transfer is shown by the arrows in each Hfr strain. Distances in minutes are shown. Leu: leucine biosynthesis; nadD: NAD biosynthesis; pyrC: pyrimidine biosynthesis; trp: tryptophan…
You are studying a type of bacteria isolated from the acidic water runoff of a mining operation. You subject two batches of the same bacteria type to different environmental growth conditions. One batch is grown at pH 2, while the other is grown at pH 7. All other environmental parameters are kept identical between the two batches. You then collect their proteins and run a Western blot using an antibody that binds to a proton efflux pump protein (which actively expends energy to pump protons out of a cell). How would you characterize the information obtained in this experiment? What does it tell you, and why is that potentially valuable information?
a bacteriophage has been isolated from the growth chambers that has evolved the capability to the methylate its own DNA when it is replicated inside the host bacterium. why is this a problem?

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