ii A thermophillic bacterium was isolated from a hot spring at Perak to study its thermostableamylase gene. Before the gene could be cloned for further work, the genome of this bacteriashould first be extracted.(i)State FOUR (4) main methods you may use to lyse the cells prior to DNAisolation. Give ONE (1) example of each method.After the genome is obtained, explain in detail how the amylase gene could be clonedinto Escherichia coli competent cells to be amplified.(ii)

Lysis is the breaking down of the membrane of a cell and allow it to lose its contents.

nylase gene. Before the gene could be cioned for fürther work, the genome of this bacteria hould first be extracted. State FOUR (4) main methods you may use to lyse the cells prior to DNA isolation. Give ONE (1) example of each method. i) After the genome is obtained, explain in detail how the amylase gene could be cloned into Escherichia coli competent cells to be amplified.

nylase gene. Before the gene could be cioned for fürther work, the genome of this bacteria hould first be extracted. State FOUR (4) main methods you may use to lyse the cells prior to DNA isolation. Give ONE (1) example of each method. i) After the genome is obtained, explain in detail how the amylase gene could be cloned into Escherichia coli competent cells to be amplified.

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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