7. You are preparing a library to sequence two samples, one from a WS and one from a FS, via Illumina. After tagmentation, you perform the PCR using a pair of sequencing primers, which consists of a forward and a reverse primer, as described in lecture. Unfortunately, you used the same pair of sequencing primers to both samples, instead of a unique pair to each of them. Briefly explain how and why this change is going to affect the outcome. Hint: in other words, what it will happen when the software will try to align the reads after sequencing?
7. You are preparing a library to sequence two samples, one from a WS and one from a FS, via Illumina. After tagmentation, you perform the PCR using a pair of sequencing primers, which consists of a forward and a reverse primer, as described in lecture. Unfortunately, you used the same pair of sequencing primers to both samples, instead of a unique pair to each of them. Briefly explain how and why this change is going to affect the outcome. Hint: in other words, what it will happen when the software will try to align the reads after sequencing?
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
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Transcribed Image Text:7.
You are preparing a library to sequence two samples, one from a WS and one
from a FS, via Illumina. After tagmentation, you perform the PCR using a pair of sequencing
primers, which consists of a forward and a reverse primer, as described in lecture.
Unfortunately, you used the same pair of sequencing primers to both samples, instead of a unique
pair to each of them. Briefly explain how and why this change is going to affect the outcome.
Hint: in other words, what it will happen when the software will try to align the reads after
sequencing?
Expert Solution
Step 1
Introduction
An aligned read is that sequence which has been aligned to a common reference genome.
Typically these reads can number from the hundreds of thousands to tena of millions.
- Alignments are a important way to compare affiliated DNA or protein sequences.
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