Answered: For the first modified gene sample,…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Question

Researchers are manipulating the gene cxx2 for their experiments, and they have inserted a small
number of base pairs randomly somewhere into the gene. They isolate several versions (with different
insertions) of this modified gene and carry out an RT-PCR using a primer that recognizes the
transcriptional start site area (eg. from +1 to +20), and a primer that binds to the polyA tail.
For the first modified gene sample, they observe that the intron is no longer being spliced out.
Which of the lettered arrowheads indicates a location where this insertion could be? (There
could be one answer or several. Give all answers that apply)

Here is a human gene, called cxx2. The following locations are marked: where transcription starts and
ends, the start codon and the stop codon, and the boundaries of the two exons and the one intron. The
open arrowheads (triangles) indicate specific locations in the gene. Use these locations (1-10) to answer
the following questions.
1
2
transcriptional
start site
+1
3
Ĵ
ATG
exon/intron intron/exon
boundary boundary
intron
transcriptional
stop site
9
8
TAA
10

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