4. Draw the number of fragments as well as their sizes as they would migrate on theelectrophoresis gel. Write the size of the fragments on the square when you draw them.LadderSal IHae IIEco R1bp50,00024,00020,00016,00010,0009,0008,0007,0006,0005,0004,0003,0002,0001,000DNA migration

Sal1 is a restriction endonuclease i.e it cleaves DNA at the recognition sequence 5'-G/TCGAC-3'…

Answered: 4. Draw the number of fragments as well…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Similar questions

Use the set of gene sequencing results below to answer the question that follows: A G C T -Wells I 14. Based on the sequencing results above, what is the sequence of nucleotides as they were added by DNA polymerase to the DNA template? a/ATC-GCA-GTA b. TAG-TGC-CAT c. TAC-TGC-GAT GC-GA TAG-CGT-CAT L A EI la la اد ان YI
Hind III' Hind III .9 kb .9 kb Bam HI plasmid A .9 kb .7kb Bam HI .3 kb plasmid B .9 kb .4 kb .3 kb .5 kb/ Eco RI Bgl Il .4 kb Eco RI .7 kb .5 kb -Bgl II Bam HI Eco RI You requested that a lab run by a friend of yours share a couple of plasmids that they created with you. She was willing to send you the ones that you asked for in exchange for citation in any papers that were published using the plasmids. Eco RI However, the labels from two vials have fallen off in the shipping because the adhesive used to hold them was not able to withstand the temperature, being shipped on dry ice. Each vial contained a different plasmid and now you do not know which plasmid is in which. Fortunately, you have restriction maps for both plasmids, shown above. Explain how you would determine which vial contained each plasmid and give a graphical representation of your expectations related to your method of Bam HI discovery. (25pts)
4. A linear piece of DNA has the following EcoRI restriction sites. EcoRI site 1 2 kb 4 kb EcoRI site 2 5 kb a. This piece of DNA is cut by EcoRI, the resulting fragments are separated by gel electrophoresis, and the gel is stained with ethidium bromide. Draw a picture of the bands that will appear on the gel.
Did your recombinant DNA have more than one insert? What was the orientation of the insert(s)? Make a rough map of yoir recombinant plasmid. (Edvotek Recombinant DNA Lab Module V)
Can you explain each of the statement given i didnt really understamd the dna recombinant
Choices: Origin of replication Bubble SSBP RPA Sliding Clamp PCNA DNA Pol III Pol ε Pol δ DNA Pol I RNAse H Flap 1 DNA gyrase Pol α DNA helicase Primase Single chromosome Multiple points in chromosomes DNA ligase Not applicable 7. Adds more nucleotides in the leading strand of prokaryotes 8. Make primers for the replicative enzymes in eukaryotes 9. Primosome in prokaryotes 10. Removal of prokaryotic primer 11. Adds more nucleotides in the leading strand of eukaryotes
y Cours S DIOE The following DNA fragment shows where a number of restriction endonucleases cut sites occur within the fragment. The numbers below indicate the distance between those cut sites in base pairs (bp). Kpn I Sall Eco RI Pst Bam HI Xho Bam HI Pst i Eco RI Kon I 100 200 300 400 500 B00 700 800 900 1000 1100 1200 1300 1400 1500 1600 1700 1800 1900 2000 How many total restriction fragments will result if you complete digest the DNA fragment above with Sal I? cross out a. 1 cross out b. 2 cross out O c. 3 cross out d. 4 cross out e. 5 cross out O f. 6 cross out g. 7 cross out Oh. 8 The following DNA fragment shows where a number of restriction endonucleases cut sites occur within the fragment. The numbers below indicate the distance between those cut sites in base pairs (bp). t of Sal Eco RI Pat Xhol Bam HI Psti Eco RI Koni Kpn i Bam H
4. A 12 kb linear DNA fragment is subject to single or double RE digest and agarose gel electrophoresis, to yield the gel profile shown below. The first lane contains the size marker (M). M Undigested EcoRI control PvuII ECORI + PvuI 12000 bp 9000 bp 6000 bp 5000 bp 4000 bp 2000 bp 1000 bp Explain how the name of the enzyme ECORI is derived. How many sites are there for EcoRI and PvuII respectively on this DNA fragment? b) c) Use the sizes of the DNA bands on the gel to compile a restriction enzyme map of the DNA fragment. Indicate the positions of the restriction enzymes sites for EcoRI and PvuII on the map.
Give the DNA compliment to the following DNA strand. CTA DNA a BTW GAT СТА
9. During nucleic acid hybridization, the probe is labelled for DNA stability to increase probe-test DNA binding to identify the location of probe and the test DNA binding for amplification 11. What is not true for Sequence tagged site (STS) markers: cannot be mapped by fluorescence in situ hybridization (FISH) subset of STS markers are known as expressed sequence tag (EST) markers can readily be screened by a PCR assay short DNA sequences that occur at a unique location in the genome
In the figure, which ketter represents DNA polymerase 1 D AA BB C.C DD EE
Section B: Circular DNA Eco01091 2698 Pfol 46 BSTAPI 179 Aatll 2641 Sspl 2525 Ndel 183 Ehel 235 Pdml 2318 396 MCS lacz 146 Bcgl 2239 Scal 2201 \2510 236 476 493 PUC57 2710 bp BsaXI 683 Sapl 707 876, AfillI, Pscl 830 Gsul 1808 Cfr101 1803- Eco311 1790 Eam11051 1718 1650 1490 rep (PMB1) \BseYl 1134 \Cail 1241 te fenen P S GTAA AAC GAC Goc CA TGA ATT CGA GCT CGG TAC CTc cCG AAT GCA TCT AGA TAT CGG ATC ccG Gạc ccG TCG ACT GCA GAG GCC TGC ATG CAA GCT Too 3' CATT TO Cra cco orC ACT TAA OCT COA GCC ATG GAG cOC TTA COT AGA TCT ATA GCC TAG 0oc coa 00C AGC TGA COT CTC c00 ACO TACOTT COA Ace Lad Va V Al Le Se Aen Ser Se P Val C Arn e Cy Ag Ser e Pe A Arg Als Arg Arg Se O Le ay Al His Le Ser e caT AAT CAT Gar CAT AGC Tor Tre era 3 OCA TTA GTA CCA GTATOSACA AAG GAC S The le Met The Met 1. You have a circular plasmid (PUC57), which consists of 2710 bp. What would the number and length of the fragments be if you cut the plasmid with the following restriction enzymes or a combination of enzymes?. Show…

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