B. 25mL of 1% Sodium Bicarbonate solution was created and the ph was taken. Whicvalue below represents the most accurate representation fo the pH of the solution?a.pH 8.5b. pH 7.0c. pH 6.58.Agarose gel electrophoresis was performed below.Lane 1 is a 100 base pair DNA ladder.1 2 3Divong 20dut arinat & bakA. Does Lane 2 or lane 3 show a larger DNA fragment?B. Given the Lane 1 DNA ladder sizes, what is theapproximate size (in base pairs) of the amplicon in Lane3?

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Answered: 8. B. 25mL of 1% Sodium Bicarbonate…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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4. In bacterial cells, nucleotide excision repair involves which of the following proteins?A. DNA glycosylaseB. AP endonucleaseC. photolyaseD. AlkBE. UvrABC proteins 5. If Meselson and Stahl had results from density gradient analysis of bacterial DNA that indicated only two bands, one of the original density and one that was the same as unlabeled DNA, and no intermediate density band, this would indicate that DNA replication is:A. constructiveB. semiconservativeC. conservativeD. consecutiveE. cannot determine from the information given 6.In E. coli, which DNA polymerase is primarily responsible for filling in the gaps in the DNA generated during nucleotide excision repair?A. DNA polymerase IB. DNA polymerase IIC. DNA polymerase IVD. DNA polymerase VE. none of the above
do C and it sub part pls
A recombinant DNA contains -- and Which of the following terms is appropriate for the blanks? Lütfen birini seçin: O a. gene to be cloned / cell O b. plasmid / bacterial DNA c. gene to be cloned/enzyme O d. plasmid / gene to be cloned O e. plasmid / protein
2. Please help answer this question and show all work, thank you so much.
1. Why were nuclei being unstained when you used eosin? 2. What is the relationship between heterochromatin and the synthetic activity of DNA? 3. What is the significance of using different histological stains? 4. Aside from H&E, what other staining methods are applicable in Golgi apparatus, mitochondria, and nucleic acid? What are the differences? 5. How essential is Periodic Acid- Schiff Reagent? please answer with 3-5 sentences
4. After reading the lab for next week, use the following simulated DNA gel to answer the questions. a. Label the bands of the two-log ladder with the lowest band as the lowest number of kilobases (far left column or lane) b. Draw an arrow indicating the direction the DNA fragments travelled c. Estimate the number of kilobases in the unknown DNA in lanes 7 and 8 MW 1 2 3 4 5 6 7 8
Bradford Assay: Coomassie Blue G-250, phosphoric acid: A. Biuret Protein Assay: sodium potassium tartrate, sodium carbonate, sodium hydroxide, copper (II) sulfate, Folin-Ciocalteau B. Lowry Assay: sodium hydroxide, copper (II) sulfate, sodium potassium tartrate. C. Bicinchoninic Acid Assay: bicinchoninic acid, sodium carbonate, sodium bicarbonate, sodium potassium tartrate, copper (II) sulfate D. Both A and B E. All of the above
OO HUAWEI Nova 2 Plus DUAL CAMERA tem (Academic) c. Heat shock. d. Floral dip. O e. Electrophoresis. The hybridization between DNA during the library screening process take place between probs and CDNA of the organism when: Select one: O a. Van der Waals forces. O b. Hydrogen bonds form between the complementary DNA sequences. O c. Covalent bond form between the complementary the DNA sequences. O d. Glycosidic bonds. e. Phosphodiester bond and cause ligation the DNA sequences. Next
Explain why a solid state fermentation system is optimal for extracellular enzyme production from fungi? 7. You have discovered a secondary metabolite made by an unusual fungus that has great industrial potential. Unfortunately, the fungus makes very of this product, so you must velop a scheme to increase the yield. Can you think of using a technique that might be useful to enhance the productivity of that secondary metabolite?
Hind III' Hind III .9 kb .9 kb Bam HI plasmid A .9 kb .7kb Bam HI .3 kb plasmid B .9 kb .4 kb .3 kb .5 kb/ Eco RI Bgl Il .4 kb Eco RI .7 kb .5 kb -Bgl II Bam HI Eco RI You requested that a lab run by a friend of yours share a couple of plasmids that they created with you. She was willing to send you the ones that you asked for in exchange for citation in any papers that were published using the plasmids. Eco RI However, the labels from two vials have fallen off in the shipping because the adhesive used to hold them was not able to withstand the temperature, being shipped on dry ice. Each vial contained a different plasmid and now you do not know which plasmid is in which. Fortunately, you have restriction maps for both plasmids, shown above. Explain how you would determine which vial contained each plasmid and give a graphical representation of your expectations related to your method of Bam HI discovery. (25pts)
1c) T. aquaticus genomic DNA is 34.3% guanosine nucleotides. What fraction of the DNA is adenosine nucleotides? 1 0.9 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 Fraction of DNA
2. A. B. C. The DNA of each species has a different base composition. Find the base composition of each species, using what you know about complementary base pairing. Human DNA is approximately 20% C. G = % % A = % In the bacteriophage DX174, the DNA is approximately 26% A. C = % G = % A = T= Sea urchin DNA is approximately 18% G. C = % T = % T= % % 64

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