B. 25mL of 1% Sodium Bicarbonate solution was created and the ph was taken. Whicvalue below represents the most accurate representation fo the pH of the solution?a.pH 8.5b. pH 7.0c. pH 6.58.Agarose gel electrophoresis was performed below.Lane 1 is a 100 base pair DNA ladder.1 2 3Divong 20dut arinat & bakA. Does Lane 2 or lane 3 show a larger DNA fragment?B. Given the Lane 1 DNA ladder sizes, what is theapproximate size (in base pairs) of the amplicon in Lane3?

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Answered: 8. B. 25mL of 1% Sodium Bicarbonate…

Concepts of Biology

1st Edition

ISBN:9781938168116

Author:Samantha Fowler, Rebecca Roush, James Wise

Publisher:Samantha Fowler, Rebecca Roush, James Wise

Chapter10: Biotechnology

Section: Chapter Questions

Problem 2RQ: n gel electrophoresis of DNA, the different bands in the final gel form because the DNA molecules...

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Why is the company Qiagen has more refined DNA extraction steps than a normal Strawberry DNA extraction practical? Summary of Qiagen DNA extraction steps Add ATL buffer and grind with sample. Add 20 microliters of enzyme Proteinase K to degrade protein into a 1.5-2ml microcentrifuge tube. Add 200 microlitres AL lysis buffer, and mix by vortexing for 5–10 seconds, which breaks cell membrane allowing DNA to be released. Incubate the sample at 56 degrees for 10 minutes. Mix the cell lysate with 200 microlitres ethanol by pipetting it at the side of the microcentrifuge wall so DNA precipitates. The DNA forms a white layer and the remaining liquid is discarded. Pipet the mixture into DNeasy Mini spin column placed in a 2 ml collection tube. Centrifuge for a minute at 8000 rpm. Place the mini spin column into a 2 ml collection tube, add 500 µl Buffer AW1, and centrifuge for 1 min at 8000 rpm. Then add it to a new 2 ml collection tube (provided), add 500 µl Buffer AW1, and centrifuge for 1…
d. Biuret test O e. NaOH Clear my choice In the method to isolate the DNA from peas, you used the centrifuge right after the addition of salts and alcohol; in which part of the tube will you find the stringy white DNA? of Select one: stion a. Suspended in the solution O b. At this step, no DNA will be visible O c. Top of the tube precipitate O d. Bottom of the tube precipitate Clear my choice
4. Look at the gel image and answer the questions below and be specific. a) Based on your calculations of the DNA concentrations, how much DNA was loaded into each well? Do you see DNA for each of your samples? If not, why do you think that is so? b) Is the DNA in a single sharp band, multiple bands or a smear? What would each of these scenarios be due to, and why would you see them for your samples? c) Do you see multiple bands in your plasmid DNA sample? What are they?
The chromatogram shows fluorescent peak data from a dye-terminating nucleotide-sequencing reaction. The peaks are shown with shortest fragment on the left to longer fragments on the right. T •C A Select the DNA sequence that matches the data. 5-ТАТAСТТАСGAAGT-3' 5'-GTCCTACGGACGCG–3' 5'-ATATGAATGCTTCA–3' 5'-TGAAGCATTCATAT–3' 5-АСТТCGTAAGTATA-3'
Question Image hown bele nallest? Q. A gel from gel electrophoresis is shown below. Which DNA fragment is the smallest? B. Direction of Travel
You have two sewage samples that you want to check for the SARS Cov 2 viral load. Whichvariable would give you a good estimate?a. High Ct valueb. Low Ct valuec. High melting temperatured. Low melting temperaturee. None of the above The DNA fragments generated during a cycle sequencing reaction are separated by sizes usingcapillary gel electrophoresis. The determination of the actual sequence is based on detection ofa. fluorescently labelled ddNTPsb. the lengths of the fragmentsc. fluorescently labelled probesd. fluorescently labelled primers.
Sanger DNA sequencing/ dideoxy sequencing was used as shown in the diagram below. The arrow indicates the direction for migration of the bands in the gel. What are the first 3 letters of this DNA sequence?
The rate of migration of DNA within an agarose gel in the gel electrophoresis technique is dependent on what factor(s)? Select one or more: a. G-C /A-T ratio b. Well size of the gel c. Size of DNA fragment d. Volume of sample loaded e. Negative charge of DNA
4. You isolate DNA from 1 ml of a suspension of E. coli cells using the procedure outlined in your lab handout. After completing the DNA isolation procedure, the total volume of the DNA solution is 700 µul. You transfer 80 µl of this DNA solution into 720 µl of TE buffer to create a diluted DNA solution. Using a spectrophotometer, you determine that the concentration of the diluted DNA solution is 440 µg/ml. a. How much did you dilute the original DNA solution? Express the dilution as a ratio. b. What is the concentration of the original DNA solution? c. How much total DNA did you isolate from the suspension of E. coli cells? d. How much total DNA was present in the diluted DNA solution?
1. Draw a gel showing the bands/fragments generated from cutting the plasmid below with Sall, BamHI and Pstl. Make sure you label the bands with the expected sizes. PstI 3607 3000 4000 amp ori HindIII EcoRI 4359 0 29 EcoRV 2295 1 NdeI 185 pBR322 4361 bp 375 tet 2000 BamHI 651 Sall 1000 2. If you want to use the plasmid above to clone an insert of 500 bp using BamHI and Sall, what would your expected FINAL plasmid+insert size to be if your cloning was successful? (remember that you first have to treat the intact pBR322 with BamHI and Sall to prepare it for introducing the insert).
1. You are given a tube containing 1.5 ml of a DNA solution. The concentration of the DNA solution is 1.2 µg/ul. a. What is the concentration of the DNA solution in mg/ml? b. How much total DNA is present in the DNA solution? Give the mass of DNA in both ug and mg.
When gel electrophoresis is undertaken, one well always contains DNA-marker molecules. The purpose of these markers is to Question 30 options: help visualize the DNA indicate when the DC current is turned on serve as a guide to the length of the fragments in the other wells indicate when the DNA has completely crossed the gel serve as a channel for ethidium bromide

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