4. A peptide was cleaved into smaller fragments using chymotrypsin and CnBr. The fragments were separated by HPLC and then sequenced by Edman degradation. Determine the sequence of the original peptide from the following fragment sequences. Cleavage with chymotrypsin Cleavage with CnBr EAGPDGMECAF ЕCАFHR GPF CKWEAGPDGM EAAMCKW IAHTYGPFEAAM HR ТАНТY -align the peptides Reagent (source) Trypsin (bovine pancrease) Chymotrypsin (bovine pancrease) Staphylococcus V8 protease Pepsin (porcine pancrease) Cyanogen bromide (chemical)(CnBr) Specificity Lys, Arg (C) Phe, Trp, Tyr (C) Glu, Asp (C) Phe, Trp, Tyr (N) Met (C)
4. A peptide was cleaved into smaller fragments using chymotrypsin and CnBr. The fragments were separated by HPLC and then sequenced by Edman degradation. Determine the sequence of the original peptide from the following fragment sequences. Cleavage with chymotrypsin Cleavage with CnBr EAGPDGMECAF ЕCАFHR GPF CKWEAGPDGM EAAMCKW IAHTYGPFEAAM HR ТАНТY -align the peptides Reagent (source) Trypsin (bovine pancrease) Chymotrypsin (bovine pancrease) Staphylococcus V8 protease Pepsin (porcine pancrease) Cyanogen bromide (chemical)(CnBr) Specificity Lys, Arg (C) Phe, Trp, Tyr (C) Glu, Asp (C) Phe, Trp, Tyr (N) Met (C)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:4. A peptide was cleaved into smaller fragments using chymotrypsin and CnBr. The
fragments were separated by HPLC and then sequenced by Edman degradation.
Determine the sequence of the original peptide from the following fragment sequences.
Cleavage with chymotrypsin
Cleavage with CnBr
EAGPDGMECAF
ЕCAFHR
GPF
CKWEAGPDGM
EAAMCKW
IAHTYGPFEAAM
HR
ТАНТY
-align the peptides
Reagent (source)
Trypsin (bovine pancrease)
Chymotrypsin (bovine pancrease)
Staphylococcus V8 protease
Pepsin (porcine pancrease)
Cyanogen bromide (chemical)(CnBr)
Specificity
Lys, Arg (C)
Phe, Trp, Tyr (C)
Glu, Asp (C)
Phe, Trp, Tyr (N)
Met (C)
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