Two complete sets of overlapping peptides, created with two different cleavage methods, areused in protein sequencing primarily because:O the sequencing methods are inaccurateO a single cleavage method do not give identical peptidesO amino acids at the end of peptides are often degradedO the sequence information deteriorates with peptide lengthO the overlaps are needed to properly order and align the various peptides

The technique used to determine the amino acid sequence of a protein is known as protein sequencing.…

Answered: Two complete sets of overlapping…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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What is meant by the term "alignment" in genomic research and in what ways does alignment aid sequence assembly? How can relying on alignment lead to potential errors?
b) A gene was isolated from mouse and sequenced by a research group. When the sequencing result was obtained, the research leader requested her assistant to generate the results below (Figure 1) using a Bioinformatics tool. >>> Descriptions Graphic Summary Sequences producing significant alignments select all 200 sequences selected Alignments Taxonomy Mus musculus itin 1(Sit1), transcript variant 1. mRNA Mus musculus Siapha(S)RNA completed Mus musculus Sic2alpha orain IS2alphai mRNA complete ods PREDICTED: Mus.caroll situin 1(Sirt1) transcript variant X1. mRNA ✔Mus musculus situin 1(S1), transcript variant 2.mRNA ✔PREDICTED: Anvicanthis niloticus situin 1 (Sirt1) transcript variant X1 mRNA PREDICTED: Acanthis nicticus siruin 1.(Sirt1) transvaatX2 NA (i) Description (ii) Download ✔Mus musculus mutent stem cell CRL-2070 NE cDNA. RIKEN M-length enriched library, clone:G430107915 productsituin 10sent: 0030 6805 95% PREDICTED:Mastommys.coucha sittuin 1 (Sirt1), transcript variant X1. mRNA…
A polypeptide is cleaved into peptides by treatment with trypsin and cyanogen bromide, and then the peptides are purified and sequenced. The sequences of the peptides are shown below. Trypsin peptides Cyanogen bromide peptides Based on sequences of the overlapping peptides generated by treatment with trypsin and cyanogen bromide (shown above), which of the following peptides represents the N-terminus of the original polypeptide? A. T-1 B. T-4 C. C-2 D. C-4 T-1 FENYAT-2 ELIMVPKT-3 NFEEGSKT-4 ITGLAIHQKT-5 GSMDPVALMTLR C-1 DPVALMC-2 VPKGSMC-3 ITGLAIHQKELIMC-4 TLRNFEEGSKFENYA
Below is an EMSA showing four different reactions, A-D. In each tube there is some combination of labelled DNA probe, Protein X (the protein you are studying), and an antibody for Protein X. Identify which combination of components are found in each of the four reactions and explain how you determined that based on the molecular interactions being studied and your knowledge of gel electrophoresis. It is possible that multiple lanes have the same component(s). A B C D EMSA
First please note this is DNA-B and NOT DNA-A, also the marked answer was wrong so please choose another one
A linear DNA molecule is subjected to complete restriction digestion by (1) EcoRI alone, (2) HindIII alone, and (3) both enzymes together. The DNA fragments are then separated using gel electrophoresis. Results are shown below: (i) (ii) (iii) EcoRI Hindill Both | | — | | 10 kb 9 kb 8 kb 5 kb 2 kb 1 kb How long is the original DNA molecule? How many EcoRI recognition sites does it have? Does the longest EcoRI fragment contain a HindIII restriction site? Explain your answer.
Damien and Jessica are friends that are interested in proteomics. One day Damien and Jessica go to a proteomics lab to have their proteome (all proteins in body) analyzed. The analysis shows that there is a difference in one amino acid within each of their hemoglobin proteins. However, both of their hemoglobin proteins appear to be functioning properly. They both come to you and ask you the significance of this finding, what do you tell them? Should they be worried? Provide a detailed rationale. .
In a genome project, the following genomic DNA sequences were obtained. Assemble the sequences into a contig. Using the assembled sequence, perform a BLASTn search. Does the search produce sequences similar to your assembled sequence? 5’ TCGGGGTCCTGGGATCTCATCACTGCAGCGC 3’ 5’ACTGCAGCGCTTTCCCAGCGGGCGGTGGTAC 3’ 5’GGGCGGTGGTACTCGGGAAGTCAGGAGTGTT 3’ 5’AGGAGTGTTTAAAACCTGGGGACTGGTTTTG 3’ 5’TGGTTTTGGGGGCGCTGAAGGCAGCGCAGGA 3’
One of the most confusing aspects of determining the protein sequence that will be derived from a gene is keeping track of the polarity or directionality of each nucleotide sequence strand. Which DNA strand is identical to the mRNA transcript? Which DNA strand codes the RNA transcript?
The DNA sequence below is transcribed from left to right (the partner/coding strand is shown). Using this sequence, write the sequence of the mRNA molecule that is generated from this gene. Be sure to appropriately label the ends of the molecule. 5'-ATGCACGGCGACTAG-3' For the toolbar, press ALT+F10 (PC) or ALT+FN+F10 (Mac). B I UIS Paragraph Arial 10pt A v T 0 $1
The best molecular technique to quantify the gene transcripts is (write in full).
The amino acid sequences of peptide fragments obtained from a normal protein were compared with those obtained from the same protein synthesized by a defective gene. They were found to differ in only one peptide fragment. Their amino acid sequences are shown here: Normal: Gln-Tyr-Gly-Thr-Arg-Tyr-Val Mutant: Gln-Ser-Glu-Pro-Gly-Thr a. What is the defect in DNA?b. It was later determined that the normal peptide fragment is an octapeptide with a C-terminal Val-Leu. What is the C-terminal amino acid of the mutant peptide?

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