384 Protein Purification Methods Q4.1- What chemical properties of sodium dodecyl sulfate (SDS) contribute to equal charge/mass ratios needed for molecular mass determinations using SDS-PAGE? Why is the protein solution heat denatured prior to gel loading?

384 Protein Purification Methods Q4.1- What chemical properties of sodium dodecyl sulfate (SDS) contribute to equal charge/mass ratios needed for molecular mass determinations using SDS-PAGE? Why is the protein solution heat denatured prior to gel loading?

Biochemistry

9th Edition

ISBN:9781305961135

Author:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal

Publisher:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal

Chapter3: Amino Acids And Peptides

Section: Chapter Questions

Problem 16RE: REFECT AND APPLY Write equations to show the ionic dissociation reactions of the following amino...

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We have to deal with quantities that are extremely large or very small. For example- I may have 6,500,000,000 red blood cells, or the diameter of an iron atom is 0.000000011 inches. Numbers with many zeros can be inexpedient to work with, so scientists use scientific notation. It represents very large numbers or very small numbers in a simple form. We know that whole numbers can be extended to infinity, but we cannot write such large numbers on paper. Numbers that present in millions of places after the decimal point need to be represented simply. Thus, it is difficult to represent a few numbers in their extended form. Therefore, we use scientific notation.

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384

Protein Purification Methods

Q4.1- What chemical properties of sodium dodecyl sulfate (SDS) contribute to equal charge/mass ratios needed for molecular mass determinations using SDS-PAGE? Why is the protein solution heat denatured prior to gel loading?

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