Which protein and amino acids can it catabolize, among gelatin, urea, phenylalanine, sulfur-containing amino acias, and tryptophan? Can it desulfur proteins? Can it produce NH3 from the amino acid phenylalanine?

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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**3. Bacterium Z had the lab results below. What can you tell me about the protein metabolism of this bacterium?**

|                                | Gelatin Tubes | Urea agar Slants | Phenylalanine Slants | Peptone Iron Deeps |
|--------------------------------|---------------|------------------|----------------------|---------------------|
| **Result**                     | solid         | Fuchsia          | No color             | Blackening at butt of tube |
| **Other possible results**     | none          | none             | none                 | none                |
| **Other possible results**     | none          | none             | none                 | none                |

- Which protein and amino acids can it catabolize, among gelatin, urea, phenylalanine, sulfur-containing amino acids, and tryptophan?  
  ______________________________________________________

- Can it desulfur proteins?  
  ______________________________________________________

- Can it produce NH₃ from the amino acid phenylalanine?  
  ______________________________________________________

---

**4. Bacterium Q had the lab results below. What can you tell me about the protein metabolism of this bacterium?**

|                                | Gelatin Tubes | Urea agar Slants | Phenylalanine Slants | Peptone Iron Deeps |
|--------------------------------|---------------|------------------|----------------------|---------------------|
| **Result**                     | solid         | Fuchsia          | No color             | yellow              |
| **Other possible results**     | none          | none             | none                 | none                |
| **Other possible results**     | none          | none             | none                 | none                |

- Which protein and amino acids can it catabolize, among gelatin, urea, phenylalanine, sulfur-containing amino acids, and tryptophan?  
  ______________________________________________________

- Can it desulfur proteins?  
  ______________________________________________________

- Can it produce NH₃ from the amino acid phenylalanine?  
  ______________________________________________________
Transcribed Image Text:**Transcription:** **3. Bacterium Z had the lab results below. What can you tell me about the protein metabolism of this bacterium?** | | Gelatin Tubes | Urea agar Slants | Phenylalanine Slants | Peptone Iron Deeps | |--------------------------------|---------------|------------------|----------------------|---------------------| | **Result** | solid | Fuchsia | No color | Blackening at butt of tube | | **Other possible results** | none | none | none | none | | **Other possible results** | none | none | none | none | - Which protein and amino acids can it catabolize, among gelatin, urea, phenylalanine, sulfur-containing amino acids, and tryptophan? ______________________________________________________ - Can it desulfur proteins? ______________________________________________________ - Can it produce NH₃ from the amino acid phenylalanine? ______________________________________________________ --- **4. Bacterium Q had the lab results below. What can you tell me about the protein metabolism of this bacterium?** | | Gelatin Tubes | Urea agar Slants | Phenylalanine Slants | Peptone Iron Deeps | |--------------------------------|---------------|------------------|----------------------|---------------------| | **Result** | solid | Fuchsia | No color | yellow | | **Other possible results** | none | none | none | none | | **Other possible results** | none | none | none | none | - Which protein and amino acids can it catabolize, among gelatin, urea, phenylalanine, sulfur-containing amino acids, and tryptophan? ______________________________________________________ - Can it desulfur proteins? ______________________________________________________ - Can it produce NH₃ from the amino acid phenylalanine? ______________________________________________________
**Prelab 7 - Protein Catabolism**

In this lab, we focus on another important group of metabolism processes: the breakdown of proteins and amino acids. In these labs, we will utilize a series of media in tubes that differ in the protein for the bacteria to break down, and in the end, products they can detect. There are a few new vocabulary terms. Proteolytic enzymes are enzymes that break down proteins, usually by first breaking apart the polypeptide into individual amino acids. Hydrolysis reaction is used to break apart the peptide bond holding the amino acids in the polypeptide together. Hydrolysis is used to break down urea, a compound produced by the body from the destruction of proteins.

Once broken down into individual amino acids, often the amine (NH₂) functional group is broken off one end in a deamination reaction, creating NH₃. In a decarboxylation reaction, the carboxyl group is removed, creating CO₂. In a desulfuration reaction, a sulfur is removed. Examples of these processes can be found in figures in the lab background information.

1. The end product of protein metabolism is often ammonia, a base. What effect will this have on pH, will it go up or down?
   - [space for student input]

2. Another common end product, pyruvic acid. Into which metabolic pathway will the body use pyruvic acid as a substrate to produce ATP?
   - [space for student input]

To become familiar with the media before lab, please read both protein catabolism exercise background information. In particular, pay attention to the figures. Similar to last lab, the table below summarizes the different metabolic tests. Using the table, interpret the results in the following questions. There are results to interpret on the back of this handout.

|                           | **Gelatin Tubes** | **Urea Agar Slants** | **Phenylalanine Slants** | **Peptone Iron Deeps** |
|---------------------------|-------------------|----------------------|--------------------------|-----------------------|
| **Protein or Amino Acid in media** | Gelatin protein    | Urea                 | Phenylalanine            | Peptone               |
| **Reagent added in second period** | none              | None                 | Iron (III) chloride (Fe³⁺) | none                  |
| **Initial color**          | no color            | orange               | No
Transcribed Image Text:**Prelab 7 - Protein Catabolism** In this lab, we focus on another important group of metabolism processes: the breakdown of proteins and amino acids. In these labs, we will utilize a series of media in tubes that differ in the protein for the bacteria to break down, and in the end, products they can detect. There are a few new vocabulary terms. Proteolytic enzymes are enzymes that break down proteins, usually by first breaking apart the polypeptide into individual amino acids. Hydrolysis reaction is used to break apart the peptide bond holding the amino acids in the polypeptide together. Hydrolysis is used to break down urea, a compound produced by the body from the destruction of proteins. Once broken down into individual amino acids, often the amine (NH₂) functional group is broken off one end in a deamination reaction, creating NH₃. In a decarboxylation reaction, the carboxyl group is removed, creating CO₂. In a desulfuration reaction, a sulfur is removed. Examples of these processes can be found in figures in the lab background information. 1. The end product of protein metabolism is often ammonia, a base. What effect will this have on pH, will it go up or down? - [space for student input] 2. Another common end product, pyruvic acid. Into which metabolic pathway will the body use pyruvic acid as a substrate to produce ATP? - [space for student input] To become familiar with the media before lab, please read both protein catabolism exercise background information. In particular, pay attention to the figures. Similar to last lab, the table below summarizes the different metabolic tests. Using the table, interpret the results in the following questions. There are results to interpret on the back of this handout. | | **Gelatin Tubes** | **Urea Agar Slants** | **Phenylalanine Slants** | **Peptone Iron Deeps** | |---------------------------|-------------------|----------------------|--------------------------|-----------------------| | **Protein or Amino Acid in media** | Gelatin protein | Urea | Phenylalanine | Peptone | | **Reagent added in second period** | none | None | Iron (III) chloride (Fe³⁺) | none | | **Initial color** | no color | orange | No
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