Determination of MW by Gel electrophoresis
Proteins
We generally tend to think of proteins only from a dietary lens, as a component of what we eat. However, they are among the most important and abundant organic macromolecules in the human body, with diverse structures and functions. Every cell contains thousands and thousands of proteins, each with specific functions. Some help in the formation of cellular membrane or walls, some help the cell to move, others act as messages or signals and flow seamlessly from one cell to another, carrying information.
Protein Expression
The method by which living organisms synthesize proteins and further modify and regulate them is called protein expression. Protein expression plays a significant role in several types of research and is highly utilized in molecular biology, biochemistry, and protein research laboratories.
Determination of MW by Gel electrophoresis
 alt="2.7 Problem in the Determination of Molecular Welght by Gel Electrophoresis A puri-
fied recombinant protein is analyzed for molecular weight by SDS-PAGE at pH 8.5. From
the protein sequence deduced from the gene that was expressed in bacteria, the protein is
expected to have a molecular weight of 44,000. However, the molecular weight of the pro-
tein is found by SDS-PAGE to be 52.000. Explain the reason or reasons for this difference
in molecular weight. What calculation could you make to help explain this discrepancy?
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