Determination of MW by Gel electrophoresis

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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Determination of MW by Gel electrophoresis

2.7 Problem in the Determination of Molecular Welght by Gel Electrophoresis A puri-
fied recombinant protein is analyzed for molecular weight by SDS-PAGE at pH 8.5. From
the protein sequence deduced from the gene that was expressed in bacteria, the protein is
expected to have a molecular weight of 44,000. However, the molecular weight of the pro-
tein is found by SDS-PAGE to be 52.000. Explain the reason or reasons for this difference
in molecular weight. What calculation could you make to help explain this discrepancy?
You ora dorionino 1 mini vertical
Transcribed Image Text:2.7 Problem in the Determination of Molecular Welght by Gel Electrophoresis A puri- fied recombinant protein is analyzed for molecular weight by SDS-PAGE at pH 8.5. From the protein sequence deduced from the gene that was expressed in bacteria, the protein is expected to have a molecular weight of 44,000. However, the molecular weight of the pro- tein is found by SDS-PAGE to be 52.000. Explain the reason or reasons for this difference in molecular weight. What calculation could you make to help explain this discrepancy? You ora dorionino 1 mini vertical
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