Peptides & X-ray/NMR Methods Q4.2- Describe one advantage and one disadvantage of trying to solve molecular protein structures by 1) X-ray crystallography and by 2) Nuclear Magnetic Resonance (NMR) spectroscopy.
384
Peptides & X-ray/NMR Methods
Q4.2- Describe one advantage and one disadvantage of trying to solve molecular protein structures by 1) X-ray crystallography and by 2) Nuclear Magnetic Resonance (NMR) spectroscopy.
1) X-ray crystallography - is a method of solving the protein structure using the X-ray beam and protein crystal. In this method, protein is crystallized near its pI range, just before its precipitation. Good quality of protein crystal is diffracted by beam of incident X-rays into many specific directions. Then, from the electron density, the mean positions of the atoms in the crystal can be determined, and also chemical bonds, groups and other important information related to its structure can be determined by data analysis of its diffraction pattern.
2) Nuclear Magnetic Resonance (NMR) spectroscopy - is a type of spectroscopic method in which interaction of radiofrequency (Rf) electromagnetic radiations with the nuclei of molecules were recorded under strong magnetic field. NMR signal is detected with sensitive radio receivers. The intramolecular magnetic field around an atom in a molecule changes the resonance frequency significantly, this gives the details of the electronic structure of that molecule along with its functional groups.
Step by step
Solved in 2 steps
