From the following information determine the amino acid sequence of a peptide. N-terminal Edman gives PTH-Alanine C terminal carboxypeptidase treatment, no observable reaction Trypsin cleavage gives three products Arg Peptide containing Ala, Lys Peptide containing Asp, Met, Phe, Pro Mild Chymotrypsin cleavage gives 2 peptides Peptide containing Asp, Pro Peptide containing Ala, Arg, Lys, Met, Phe CNBr cleavage gives 2 peptides Peptide containing Ala, Arg, Lys and homoserine Peptide containing Asp, Phe, Pro You must supply the answer as the 3-letter amino acid sequence from N-terminus to C-terminus in the form (you must use dashes, not spaces between the amino acids)
Proteins
We generally tend to think of proteins only from a dietary lens, as a component of what we eat. However, they are among the most important and abundant organic macromolecules in the human body, with diverse structures and functions. Every cell contains thousands and thousands of proteins, each with specific functions. Some help in the formation of cellular membrane or walls, some help the cell to move, others act as messages or signals and flow seamlessly from one cell to another, carrying information.
Protein Expression
The method by which living organisms synthesize proteins and further modify and regulate them is called protein expression. Protein expression plays a significant role in several types of research and is highly utilized in molecular biology, biochemistry, and protein research laboratories.
Problem: From the following information determine the amino acid sequence of a peptide.
N-terminal Edman gives PTH-Alanine
C terminal carboxypeptidase treatment, no observable reaction
Trypsin cleavage gives three products
- Arg
- Peptide containing Ala, Lys
- Peptide containing Asp, Met, Phe, Pro
Mild Chymotrypsin cleavage gives 2 peptides
- Peptide containing Asp, Pro
- Peptide containing Ala, Arg, Lys, Met, Phe
CNBr cleavage gives 2 peptides
- Peptide containing Ala, Arg, Lys and homoserine
- Peptide containing Asp, Phe, Pro
You must supply the answer as the 3-letter amino acid sequence from N-terminus to C-terminus in the form (you must use dashes, not spaces between the amino acids)
Met-Thr-Glu-Trp
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
by Bartleby Expert
