4- You have isolated a peptide, which you wish to sequence. First you treat thepeptide with CNBr and isolate two fragments. In a separate test of the peptide youcleave with trypsin and obtain 3 fragments. You sequence each of the five peptidesand get the following data:Peptide 1: VALKTQAMPeptide 2: LDGKMPeptide 3: TQAMPeptide 4: LDGKPeptide 5: MVALKThe sequence of the original peptide is:

For determination of which of the 20 possible amino acids are present in a particular protein, and…

Answered: Peptide 1: VALKTQAM Peptide 2: LDGKM…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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A polypeptide is made up of ten isoleucines, then ten tyrosine, and lastly one glutamic acid. What characteristics is this peptide likely to have based on its amino acid composition?
A peptide was analyzed with the following results. Whatis the primary sequence of the peptide? Briefly state the conclusions of each experimental result and indicate your logic. a)Mass spectrometry was consistent with a peptide of 7 amino acids containing W,C,D,M,N,K, and I. b)One cycle of Edman degradation of the peptide yielded a single product, the PTH derivative of I c)Cleavage of the peptide with CNBr yielded two products. One contained 5 amino acids, and the other 2. One cycle of Edman degradation yielded the PTH derivatives of Iand N, respectively. d)Treatment of the peptide with trypsin yielded 2 products. One of them had significant light absorbance at 280 nm, and Edman degradation yielded the PTH derivative of I. The other trypsin-generated product contained 4 amino acids, and Edman degradation yielded the PTH derivative of Asp.
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Draw the structure of peptides TGPNLHGLFGR and ELAGAPPEPA, and calculate m/z values of their an, bn, and yn sequence ions.
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5) Snake venom surprisingly has been a found to be a rich source of biologically active peptides. One important class of peptides includes the bradykinin potentiating peptides which were the precursors to today's most commonly used antihypertensive drugs such as captopril or lisinopril. Let's say that you are investigating a new peptide in the bradykinin potentiating peptide class from Agkistrodon bilineatus (Mexican pit viper) venom. You suspect that the peptide has the following sequence: QWAQGRAPHPP After some harrowing experiments, you've collected and isolated a sample of this peptide. How will you confirm that the sequence is what you expected? Describe a sequence of chemical tests (at least 4) that you can do on this peptide that should give you enough information to deduce the sequence. Be sure to describe in detail each of the chemical analyses and the expected results of each.

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Peptide 1: VALKTQAM Peptide 2: LDGKM Peptide 3: TQAM Peptide 4: LDGK Peptide 5: MVALK The sequence of the original peptide is: