5) Snake venom surprisingly has been a found to be a rich source of biologically active peptides. One important class potentiating peptides which were the precursors to today's most commonly used antihypertensive drugs such as captopril or lisinopril. Let's say that you are investigating a new peptide in the bradykinin potentiating peptide class from Agkistrodon bilineatus (Mexican pit viper) venom. You suspect that the peptide has the following sequence: peptides includes the bradykinin QWAQGRAPHPP After some harrowing experiments, you've collected and isolated a sample of this peptide. How will you confirm that the sequence is what you expected? Describe a sequence of chemical tests (at least 4) that you can do on this peptide that should give you enough information to deduce the sequence. Be sure to describe in detail each of the chemical analyses and the expected results of each.

Q: Can you please help find what Glutamate is?

A: Amino acids are the smallest entities that make up the proteins or peptides via peptide bond…

Q: Peptide ionization Consider a peptide with the sequence Ser-His-Glu-Cys-Arg-Gln. You will be asked…

A: The pKa of an amino acid that resides within a protein refers to the pKa of the side chain of that…

Q: Which one of the following would best allow us to conclude that the rate of chymotrypsin was…

A: Chymotrypsin is a serine protease enzyme that aids in digestion in several organisms including…

Q: Identify and briefly describe three different PEGylation strategies you can use to PEGylate this…

A: The process of PEGylation improves drug solubility and decreases immunogenicity of a molecule that…

Q: How do you think incorporating b-homoserine into a peptide would affect the structure and/or…

A: Homoserine, is the product of a cyanogen bromide cleavage of a peptide by degradation of methionine.…

Q: His-Met-Asp-Tyr-Phe-Ser Calculate an approximate pI (isoelectric point) for this peptide. Please use…

A: Recall that: Amino acid sequences are written with N-terminal amino acid on the left and C-terminal…

Q: Is this the correct answer of finding what the charge is?

A: Amino acids are the smallest units that make up the peptides or proteins inside the cell. Twenty…

Q: Tricyclic, anti-depressant drugs were developed from the observation that some tricyclic,…

A: Tricyclic antidepressants (TCAs) and tricyclic antipsychotics share a common tricyclic structure,…

Q: Based on this report (and assuming that none of the compounds significantly increased inflammation),…

A: The scientists have isolated there compounds and are checking out its efficacy in mice. The muscle…

Q: Crystal structures exist for three neurokinin-1 (NK1) ligand complexes with the following pdb codes…

A: NK1 is a type of mammalian tachykinin receptors. These are G-protein coupled receptors which are…

Q: (a) The diagram below on the right-hand side illustrates the polar Asp(a94)... Asn (102) interaction…

A: A table summarizing the characteristics of many hemoglobin mutants affecting the 94 position is…

Q: Regions of 2 receptors that interact on dimerization are shown below. Identify the types of…

A: Answer: Introduction:Multiple amino acids are connected by different bonds like peptide bonds and…

Q: Based on how data is collected in the techniques of X-ray crystallography compared to NMR, the…

A: X-ray crystallography uses X-rays to determine the structure of a molecule. NMR uses strong local…

Q: Consider the following Peptide: Alanine-Lysine-Glutamine-Serine-Glycine Select all that applies Give…

A: Peptides are short chain of amino acids linked by peptide bonds. Amino acids that have been…

Q: "Regarding resolution of mass spectrometers and various ways mass of peptides can be represented,…

A: Mass spectrometry is a measured charge to mass ratio.

Q: Synthesis of peptide bonds is [exorgonic or endergonic]. Thus in isolation, this reaction would…

A: Protein synthesis takes place in the ribosome. It required the hydrolysis of GTP. The translation is…

Q: Which of the peptide sequences below best matches the hydropathy plot shown? 5 10 15 Residue Number…

A: Hydropathy plot is a quantitative analysis of the distribution of amino acids in a protein.…

Q: At pH 1, the charge on the C-terminal carboxyl group is

A: Peptide bond: When two amino acids are joined together releasing a water molecule and forming alpha…

Q: Identify and briefly describe the different PEGylation strategies you can use to PEGylate this…

A: PEGylation refers to the process where polyethylene glycol polymer is chemically added to a protein…

Q: At pH 1, the charge on the side chain of Arg is

A: We’ll answer the first question since the exact one wasn’t specified. Please submit a new question…

Q: compare the structures of the peptide neurotransmitters Met-enkephalin and Leu-enkephalin

A: Enkephalins, which play a role in memory, learning, emotional activity, and pain, are compounds…

Q: How do you think incorporating a-methylserine into a peptide would affect the structure and/or…

A: proteins are made up of amino acids . amino acid polarity and peptide struture depends upon the side…

Q: 1. Draw the appropriate titration curve for the tripeptide Met-Lys-Val starting at pH 1 and ending…

A: Step 1: Titration Curve of Tripeptide Met-Lys-Val:To draw the titration curve, you'll need to…

Q: Subjecting this peptide to one cycle of the Edman degradation would result in L-M-F-V-W-E-Q-A-P-S-P…

A: Edman degradation was developed by Pehr Edman. In Edmam degradation the amino acid sequence of a…

Q: Explain what steps would be suitable to prepare a successful chromatographic peptide mapping?

A: Peptide mapping is used to identify primary structure of protein. Other uses of peptide mapping…

Q: Questions 1) Determine the single letter code 40 amino acid sequence of the venom peptide by…

A: Chymotrypsin (endopeptidase) hydrolyzes peptide bonds on the C-terminus of a peptide bond of an…

Q: Consider the peptide HRDMQSGAI. Part: 0/5 Part 1 of 5 Identify the side chains of the amino acid…

A:

Proteins

We generally tend to think of proteins only from a dietary lens, as a component of what we eat. However, they are among the most important and abundant organic macromolecules in the human body, with diverse structures and functions. Every cell contains thousands and thousands of proteins, each with specific functions. Some help in the formation of cellular membrane or walls, some help the cell to move, others act as messages or signals and flow seamlessly from one cell to another, carrying information.  

Protein Expression

The method by which living organisms synthesize proteins and further modify and regulate them is called protein expression. Protein expression plays a significant role in several types of research and is highly utilized in molecular biology, biochemistry, and protein research laboratories.