Answered: 3. Why is it not possible to fill all…

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Biochemistry

3. Why is it not possible to fill all the tetrahedral holes in the HCP unit cell?

Biology: The Unity and Diversity of Life (MindTap Course List)

15th Edition

ISBN:9781337408332

Author:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr

Publisher:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr

Chapter6: Where It Starts-photosynthesis

Section: Chapter Questions

Problem 12SQ: Which of the following substances does not participate in the CalvinBenson cycle? a. ATP b. NADPH c....

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6. A control phospholipid membrane is isolated in which the phospholipid tails all have an 18-C chain length and are comprised of a 50:50 mixture of saturated and unsaturated tails. In addition, about 25% of the lipids are fluorescently labeled on the head groups. a) Draw a single plot of Fluidity vs. Temperature that contains the expected curves for: Control phospholipid (line- -) Control supplemented with 18-C saturated phospholipids (dashed line - Control supplemented with 16-C chain length (dotted line • • • • • • ) b) Draw a single plot of the Fluorescence Intensity vs. Time that contains the expected curves of a Fluorescence Recovery After Photobleaching (FRAP) using the same samples as in (a) above. The FRAP experiment is conducted at the same temperature for each sample (which is the Tm of the of the control phospholipid).
5. The Cy3 and Cy5 molecules were used in a FRET experiment. (c) Draw an energy diagram for the donor and acceptor molecules showing absorbance and fluorescence transitions in each.
4. At pH 7, tryptophan crosses a lipid bilayer at about one-thousandth the rate of indole, a closely related compound: Suggest an explanation for this observation. 1
7. [10'] For a bacteriophage 77, the following data at zero concentration have been obtained: Sedimentation coefficient: $20,w = 453 S. Diffusion coefficient: D₂0. = 6.03×10-8 cm² s¹¹. 20,w Specific volume V20 = 0.639 cm³ g¹ Calculate the molecular mass M of the bacteriophage.
5. Anesthetic gases used in surgery are known to bind to the hemoglobin molecule in red blood cells. The diagram below illustrates O2 binding curves of normal human HbA in the presence of the anesthetic gas dichloromethane (DCM). O UNTREATED 100 Symbols: a Dсм 23 Torr • DCM 50 Torr x DCM I00 Torr o, 0 Torr DCM; О, 23 Torr DСМ Д, 50 Torr DСМ x, 100 Torr DCM. 80 60 40 (1 Torr = 1 mm Hg.) 20 The solutions were buffered to pH 7.4. 0.5 1.0 1.5 2.0 log p02 For the curves at 0 and 100 Torr of dichloromethane, draw their equivalent in the form of (a) (* Hill plots. Label the axes and indicate on both the plot above and the Hill plot where the value of the dissociation equilibrium constant Kd for O2 binding is defined for 0 and 100 Torr of dichloromethane Kd Hb(O2)n nO2 Hb + Be sure to label the axes, indicate the units on both axes, and make the Hill plot so that the values of Kd are equivalent to those on the saturation plot above. Indicate whether cooperativity, as indicated by the Hill…
9. Metabolically active tissues require lots of ATP, and making lots of ATP requires lots of oxygen. Luckily, metabolically active tissues have some tricks for encouraging hemoglobin to give up oxygen. Name and explain the "trick" depicted by images below. In your answer, be certain to explain the effect on hemoglobin structure. 1.0 a2 Lys 40 pH 7.6 Y 0.5- `pH 7.4 C terminus pH 7.2 Added + proton B, His 146 B, Asp 94 6. 10 po2 (kPa) Figure 7-19 Biochemistry, Sinth Edition 2ea/ H freenard Camny Figures16 Lhninge Principies of iecheminy Seventh dton 2017W.H Feenan and Company
14. Two ribosomal sub-units have sedimentation coefficients of S1 = 60 S and S2 = 40 S. Assuming that both sub-units have spherical shapes and their densities are equal, calculate an approximate ratio of their molecular weights (MW1/MW2) from their S values. SOLUTION:
3. In what direction (toward the anode, toward the cathode, or toward neither) will the following proteins move in an electric field? a. Serum albumin (pl=4.9) at pH 8.0 b. Urease (pl=5.0) at pH3.0 and pH 9.0 c. Ribonuclease (pI=9.5) at pH 4.5, 9.5 and 11.0 d. Pepsin (pl=1.0) at pH 3.5, pH7.0 and pH 9.5
Which of the following substances does not participate in the CalvinBenson cycle? a. ATP b. NADPH c. RuBP d. PGAL e. O2 f. CO2
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3. In a solution of alanine molecules, approximately what fraction of the alanine will exist as the zwitterion at pH of 2.34? What would be the average net charge of a solution of alanine molecules at pH 2.34? 50% will exist as the zwitterion and 50% will exist as the fully protonated (COOH/ NH3+) form. The average net charge of this solution would be +0.5.
1. Acid phosphatases are an important group of enzymes that can be detected in human blood serum.Under slightly acidic conditions (pH 5.0), this group of enzymes can hydrolyze biological phosphate esters as follows: R-O-P-O3-2 + H 2O  R-OH + HO-P-O3-2. Acid phosphatases are produced and can be detected in erythrocytes, kidney, spleen, the liver, and prostate gland. The enzyme from the prostate gland is clinically important because an increased activity in the blood is frequently an indication of cancer of the prostate gland. Tartrate ion can strongly inhibit the phosphatase from the prostate gland, but not acid phosphatases from other tissues. How canyou use the information above to develop a specific procedure for measuring the activity of the acid phosphatase of the prostate gland in human blood serum?