## Cytochrome c Variants in Bakers YeastIn Bakers yeast, two variants of cytochrome c, a heme-containing protein consisting of 108 amino acid residues that facilitate electron transfer reactions, have been identified. These variants, known as iso-1 and iso-2 cytochrome c, differ at 17 of the 108 amino acids. The table below details the content of proton dissociable side chains for the two cytochromes:### Table of Proton Dissociable Side Chains| Side Chain | pKa | iso-1 cytochrome c | Ziso-1 | iso-2 cytochrome c | Ziso-2 ||------------|------|--------------------|------------------|--------------------|------------------|| Arginine | 12.5 | 3 | 3 | 3 | 3 || Aspartate | 4.0 | 4 | 4 | 5 | 5 || Cysteine | 8.0 | 1 | 1 | 0 | 0 || Glutamate | 4.0 | 4 | 4 | 7 | 7 || Histidine | 6.0 | 4 | 4 | 3 | 3 || Lysine | 10.0 | 16 | 16 | 17 | 17 || Tyrosine | 9.0 | 5 | 5 | 5 | 5 || –NH3+ | 7.0 | 1 | 1 | 1 | 1 || –COOH | 4.0 | 1 | 1 | 1 | 1 |### Chromatographic SeparationThe diagram to the right illustrates the chemical groups in carboxymethyl-cellulose and DEAE-cellulose, which are used for ion exchange chromatography to purify proteins.At a pH of 8, selecting the appropriate chromatographic resin is crucial for separating iso-1-cytochrome c from iso-2-cytochrome c sourced

We are given the list and quantity of amino acid residues on the iso-1 cytochrome c and iso-2…

Answered: (b) To the right is a diagram of the…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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