3. Amniotic fluid(AF) was taken from the mother for the baby with suspected disease. Cells were counted in the amniotic fluid, the sample was placed on the Thoma slide. There were too many cells. Only 6 randomly counted on the second size of square areas; Number of cells in this AF sample found 97, 123,113, 72,60, 105. (100 µl Sample Dilluted with 200 ul Trypan Blue.) a. Average of cells = ? b. Number of cells in 1 ml=? 0.025" 0,05 1 mm Figure 1: Area Number 1: x10.000; Area Number 2: x250.000.
Microscopic examination
The analysis of minute organisms, cellular organization of any biological structure, and composition of body fluids with the help of a microscope is known as microscopic examination. The magnification of specimens or samples under study helps in attaining a clearer picture of it.
Gram Staining
Named after Hans Christian Gram, a Danish bacteriologist, Gram stain is one of the most powerful staining techniques within microbiology. This technique was introduced in 1882 to identify pneumonia-causing organisms. The Gram staining technique uses crystal violet or methylene blue as primary staining colors to distinguish gram-positive from gram-negative organisms. Under a microscope, the gram-positive organisms appear purple-brown, retaining the primary color. Gram-negative organisms appear pink or red as they do not acquire the color of the primary stain.
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