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a. The feed to the precipitation unit has 120 mg mAb/l and 28 mg impurity/l in 5 liters of product solution. Determine if 93% of the mAb can be recovered with a purity of at least 94% in the precipitation. State the concentration of each component in the solution, the mass of each component in the precipitate, volume of the solution and the mass of ammonium sulfate required. mAb solubility equation: ln S = -0.0252x + 10.2 impurity solubility equation: In S = -0.0153x +8.01 mg species I solution S = g/L ammonium sulfate b. The mAb and impurity have similar molecular weight and pl. Explain a purification step that can be used after precipitation to remove the final impurity. Describes the steps required in the process to purify the mAb.

a. The feed to the precipitation unit has 120 mg mAb/l and 28 mg impurity/l in 5 liters of product solution. Determine if 93% of the mAb can be recovered with a purity of at least 94% in the precipitation. State the concentration of each component in the solution, the mass of each component in the precipitate, volume of the solution and the mass of ammonium sulfate required. mAb solubility equation: ln S = -0.0252x + 10.2 impurity solubility equation: In S = -0.0153x +8.01 mg species I solution S = g/L ammonium sulfate b. The mAb and impurity have similar molecular weight and pl. Explain a purification step that can be used after precipitation to remove the final impurity. Describes the steps required in the process to purify the mAb.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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2. A monoclonal antibody for cancer treatment is produced in a mammalian cell plate reactor. Larger and smaller proteins and impurities are removed with gel filtration and ion exchange columns. A monoclonal antibody (mAb) will be separated from an impurity in the last few stages of purification. Waste Waste Series of gel filtration and ion - exchange columns to remove smaller and larger proteins mammalian cell Precipitation to plate reactor for of production recover and increase purity of mAb Precipitate a. The feed to the precipitation unit has 120 mg mAb/l and 28 mg impurity/l in 5 liters of product solution. Determine if 93% of the mAb can be recovered with a purity of at least 94% in the precipitation. State the concentration of each component in the solution, the mass of each component in the precipitate, volume of the solution and the mass of ammonium sulfate required. mAb solubility equation: In S = -0.0252x + 10.2 impurity solubility equation: In S = -0.0153x + 8.01 mg species S = I solution X = g/L ammonium sulfate b. The mAb and impurity have similar molecular weight and pl. Explain a purification step that can be used after precipitation to remove the final impurity. Describes the steps required in the process to purify the mAb.
Transcribed Image Text:2. A monoclonal antibody for cancer treatment is produced in a mammalian cell plate reactor. Larger and smaller proteins and impurities are removed with gel filtration and ion exchange columns. A monoclonal antibody (mAb) will be separated from an impurity in the last few stages of purification. Waste Waste Series of gel filtration and ion - exchange columns to remove smaller and larger proteins mammalian cell Precipitation to plate reactor for of production recover and increase purity of mAb Precipitate a. The feed to the precipitation unit has 120 mg mAb/l and 28 mg impurity/l in 5 liters of product solution. Determine if 93% of the mAb can be recovered with a purity of at least 94% in the precipitation. State the concentration of each component in the solution, the mass of each component in the precipitate, volume of the solution and the mass of ammonium sulfate required. mAb solubility equation: In S = -0.0252x + 10.2 impurity solubility equation: In S = -0.0153x + 8.01 mg species S = I solution X = g/L ammonium sulfate b. The mAb and impurity have similar molecular weight and pl. Explain a purification step that can be used after precipitation to remove the final impurity. Describes the steps required in the process to purify the mAb.
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