23. The mmm operon, which has sequences A, B, C, and D (which may be structural genes or regulatory sequences), encodes enzymes 1 and 2. Mutations in sequences A, B, C, and D have the following effects, where a plus sign (+) indicates that the enzyme is synthesized and a minus sign (-) indicates that the enzyme is not synthesized. mmm absent mmm pi Mutation in Enzyme Enzyme Enzyme E 2 sequence No mutation A B a. Is the mmm operon inducible or repressible? b. Indicate which sequence (A, B, C, or D) is part of the following components of the operon: Regulator gene Promoter Structural gene for enzyme 1. Structural gene for enzyme 2 + I1 + II

23. The mmm operon, which has sequences A, B, C, and D (which may be structural genes or regulatory sequences), encodes enzymes 1 and 2. Mutations in sequences A, B, C, and D have the following effects, where a plus sign (+) indicates that the enzyme is synthesized and a minus sign (-) indicates that the enzyme is not synthesized. mmm absent mmm pi Mutation in Enzyme Enzyme Enzyme E 2 sequence No mutation A B a. Is the mmm operon inducible or repressible? b. Indicate which sequence (A, B, C, or D) is part of the following components of the operon: Regulator gene Promoter Structural gene for enzyme 1. Structural gene for enzyme 2 + I1 + II

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Which area (A, B. C. or D) represents the region important for maintaining the gene of interest during binary fission? Arabinose operon Origin region ori Ndel arac Promoter Paan PGLO (5,400bp) Ndel Ampicillin Resistance Gene Amp Hindll Ndel Green Fluorescent Protein (GFP) Gene Hindll ECORI GraphicOE Schmid/2003 D O A OB OC OD
For the wildtype E. coli two sugars influence the level of transcription of the lactose operon. Fill in the table wvith the choices (shown in parenthesis) in each column to show how these two sugars influence gene regulation. Sugars in the growth media: CAMP levels positive regulation negative regulation Relative level of Lac operon transcription (highest, inbetween, lowest) (high or low) оссurs оссurs (yes or no) (yes or no) glucose present; lactose absent glucose present; lactose present glucose absent; lactose present
B Ix Paragraph Styles Dictate Sensitivit As a graduate student, Prof. Rolfes studied the E. coli regulatory protein PurR. 6. PurR regulates several small operons and single genes whose encoded proteins are involved in the biosynthesis of the purine nucleotides A and G. PurR binds to an operator (0) in the RURGMN operon to regulate its transcription. purG is a structural gene that encodes an enzyme, glutamine PRPP amidotransferase (GPAT), and its enzyme activity can be measured. The expression of purG is repressed when cells are grown in media with guanine, and that repression requires PurR (see first two lines in the table below). Predict the GPAT activity in the following strains. R refers to pwrR: O to purQ; G to purG; the + means functional and the- means non-functional. a. GPAT activity Strain Genotype R+ Q+ G+ Growth with guanine Low activity High activity Growth without guanine High activity High activity R-O+ G+ R+ O+G R+ 0-G+ R-0+ G+/F R+ R+ 0-G+/F' O+ G+ No activity No…
The organization and function of the lac operon in E. coli is shown in the following figures: XXI Transcription Repressor mRNA Translation RNA polymerase M M Flask ZYA XXXX Active M-repressor protein Repressor active, operon off. The repressor protein binds with the operator, preventing transcription from the operon. (a) An inducible operon Copyright © 2007 Pearson Education, Inc., publishing as Benjamin Cummings Allolactose (inducer) Carbon/Energy Source 0 Z MInactive repressor protein (a) An inducible operon Transcription Translation XXX Permease B-Galactosidase Transacetylase Operon mRNA 3 Repressor inactive, operon on. When the inducer allolactose binds to the repressor protein, the inactivated repressor can no longer block transcription. The structural genes are transcribed, ultimately resulting in the production of the enzymes needed for lactose catabolism. Copyright © 2007 Pearson Education, Inc., publishing as Benjamin Cummings Suppose you inoculate three flasks of minimal…
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A bacterial species has a hypothetical sigma promoter that has the following sequence: TTGGCA - 18 bases - TATAAT What change in the level of transcription would there be if the sequence was mutated to: TTCGCA -18 bases -TATAAT O The mutation would bind the promoter to the consensus and produce normal levels of transcription O The mutation would inhibit the promoter thereby inhibiting transcription The mutation would move the promoter away from consensus and reduce the level of transcription O No change the consensus TATAAT sequence in the same. D00 F3 F4 F5 F6 F7 F8 F9 % & 5 6 7 8 9 %24
1. Please tell me: What's a mutation in moleculer terms? 2. When a mutation deletes a base in genomic DNA, how does it affect the expression product production and reading frame? 3. How does induction control enzyme synthesis using lactose operon?
Where do the operator sites reside for the Lac Operon? O Upstream of promoter O Downstream of promoter O All of the options O Directly adjacent to promoter
Match each description with the appropriate label (allo)Lactose binds to the lac repressor, causing it to release from the promoter CAP binds CAMP to stimulate transcripti of the lac operon ✓ [Choose ] positive control of an inducible gene negative control of an inducible gene [Choose]