Answered: .Hanes-Woolf 2.Michaelis Menten…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question

Each plot has its own limitations. Match the plot with the correct statement on limitations.

1.Hanes-Woolf

2.Michaelis Menten

3.Lineweaver-Burk

limitations:

A. This plot appears to allow for more accurate calculation of these parameters, but is prone to error, as the y-axis takes the reciprocal of the rate of reaction –this increases any small errors in measurement at low substrate concentration, where they are most likely to occur.

B.The advantage of this plot is that it does not not overemphasizing the data obtained at low [S]. The disadvantage is that any inaccurate measures of substrate concentration will be exaggerated as [S] is used on both axes.

C.It is difficult to obtain a meaningful value of Km from this plot because of the high substrate concentrations needed to reach Vmax. Vmax has to be estimated by extrapolation- which is partly subjective and introduces error.

D.The advantage of this plot is that it overcomes uneven spacing of points, and undue weight of a low [S]. The disadvantage is that v is used on both axes and errors in measuring rates of reaction are multiplied, resulting in lower precision of estimates of km, vmax

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