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Case Study: Enzyme Kinetics Data for new statin drug (inhibits HMG CoA reductase): I Substrate (UM) 0.5 1.0 1.5 2.5 3.5 11 Rate of reaction (mm/min) 23.5 32.2 36.9 41.8 44 What are the Kms of the uninhibited and inhibited reactions? Rate of reaction in presence of 5.0 nM statin Using the above date, create a double-reciprocal (Lineweaver Burk) plot. What type of inhibition is shown by the new statin? What are the Vmax of the uninhibited and inhibited reactions? 16.67 25.25 30.49 37.04 38.91

Case Study: Enzyme Kinetics Data for new statin drug (inhibits HMG CoA reductase): I Substrate (UM) 0.5 1.0 1.5 2.5 3.5 11 Rate of reaction (mm/min) 23.5 32.2 36.9 41.8 44 What are the Kms of the uninhibited and inhibited reactions? Rate of reaction in presence of 5.0 nM statin Using the above date, create a double-reciprocal (Lineweaver Burk) plot. What type of inhibition is shown by the new statin? What are the Vmax of the uninhibited and inhibited reactions? 16.67 25.25 30.49 37.04 38.91

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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### Case Study: Enzyme Kinetics **Data for new statin drug (inhibits HMG CoA reductase):** | I | II | III | |---------------|-----------------------|--------------------------------------------| | Substrate (uM) | Rate of reaction (mM/min) | Rate of reaction in presence of 5.0 nM statin | | 0.5 | 23.5 | 16.67 | | 1.0 | 32.2 | 25.25 | | 1.5 | 36.9 | 30.49 | | 2.5 | 41.8 | 37.04 | | 3.5 | 44 | 38.91 | - **Using the above data, create a double-reciprocal (Lineweaver-Burk) plot. What type of inhibition is shown by the new statin?** - **What are the Kms of the uninhibited and inhibited reactions?** - **What are the Vmax of the uninhibited and inhibited reactions?** --- **Explanation:** This table presents data comparing enzyme reaction rates with and without the presence of a new statin drug. Column I lists substrate concentrations, while Column II and III provide the reaction rates without and with the statin, respectively. The task involves plotting this data on a Lineweaver-Burk plot to determine the type of inhibition and calculate kinetic parameters Km and Vmax for both conditions.
Transcribed Image Text:### Case Study: Enzyme Kinetics **Data for new statin drug (inhibits HMG CoA reductase):** | I | II | III | |---------------|-----------------------|--------------------------------------------| | Substrate (uM) | Rate of reaction (mM/min) | Rate of reaction in presence of 5.0 nM statin | | 0.5 | 23.5 | 16.67 | | 1.0 | 32.2 | 25.25 | | 1.5 | 36.9 | 30.49 | | 2.5 | 41.8 | 37.04 | | 3.5 | 44 | 38.91 | - **Using the above data, create a double-reciprocal (Lineweaver-Burk) plot. What type of inhibition is shown by the new statin?** - **What are the Kms of the uninhibited and inhibited reactions?** - **What are the Vmax of the uninhibited and inhibited reactions?** --- **Explanation:** This table presents data comparing enzyme reaction rates with and without the presence of a new statin drug. Column I lists substrate concentrations, while Column II and III provide the reaction rates without and with the statin, respectively. The task involves plotting this data on a Lineweaver-Burk plot to determine the type of inhibition and calculate kinetic parameters Km and Vmax for both conditions.
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