A biochemist_needs topreserve anenzyme in a 250 mL D:50 M acetate buffer at pH 5.5. (Ka of acetic accid =1.8 X 10 -5). How would the biochemist pre pare the puffer? What is the pxa. of the achtic acid ? a. -4.74 b. 1.8 d. 4.74 A bio chemist needs to preserve an enzyme io 250 mL Ka of acetic acid would the biochemist prepare the Bufferi 0-50 M acet ate buffer at pH 5.S. 1.8 X 10-5). tHow = a calculate the amount otacetic acit and todium atetate nteded-using thot pit-scate equantion b.Determine first the ratio dum-atetate t acette a cid ieeded-by estimation. ċ. Use Henderson -Hasselbalch equation to calculate the needed ratio ofujugate pasE and weak Acit. t Noñe of the given choices CS Scanned with CamScanner

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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A biochemist_needs topreserve anenzyme
in a 250 mL D:50 M acetate buffer at
pH 5.5. (Ka of acetic accid =1.8 X 10 -5).
How would the biochemist pre pare the puffer?
What is the pxa. of the achtic acid ?
a. -4.74
b. 1.8
d. 4.74
A bio chemist needs to preserve an enzyme io
250 mL
Ka of acetic acid
would the biochemist prepare the Bufferi
0-50 M acet ate buffer at pH 5.S.
1.8 X 10-5). tHow
=
a calculate the amount otacetic acit and
todium atetate nteded-using thot pit-scate
equantion
b.Determine first the ratio dum-atetate t
acette a cid ieeded-by estimation.
ċ. Use Henderson -Hasselbalch equation to
calculate the needed ratio ofujugate pasE
and weak Acit.
t Noñe of the given choices
CS
Scanned with CamScanner
Transcribed Image Text:A biochemist_needs topreserve anenzyme in a 250 mL D:50 M acetate buffer at pH 5.5. (Ka of acetic accid =1.8 X 10 -5). How would the biochemist pre pare the puffer? What is the pxa. of the achtic acid ? a. -4.74 b. 1.8 d. 4.74 A bio chemist needs to preserve an enzyme io 250 mL Ka of acetic acid would the biochemist prepare the Bufferi 0-50 M acet ate buffer at pH 5.S. 1.8 X 10-5). tHow = a calculate the amount otacetic acit and todium atetate nteded-using thot pit-scate equantion b.Determine first the ratio dum-atetate t acette a cid ieeded-by estimation. ċ. Use Henderson -Hasselbalch equation to calculate the needed ratio ofujugate pasE and weak Acit. t Noñe of the given choices CS Scanned with CamScanner
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