If inhibitor X changes lactase activity to a Vo of 0.111111111111 mM per minute when [S] = 0.40 mM, and a Vo of 0.166666666667 mM per minute when [S] = 1.0 mM, calculate the new Vmax. A. 0.10 mM per minute B. 0.25 mM per minute C. 0.50 mM per minute D. 1.0 mM per minute E. 2.0 mM per minute
Enzyme kinetics
In biochemistry, enzymes are proteins that act as biological catalysts. Catalysis is the addition of a catalyst to a chemical reaction to speed up the pace of the reaction. Catalysis can be categorized as either homogeneous or heterogeneous, depending on whether the catalysts are distributed in the same phase as that of the reactants. Enzymes are an essential part of the cell because, without them, many organic processes would slow down and thus will affect the processes that are important for cell survival and sustenance.
Regulation of Enzymes
A substance that acts as a catalyst to regulate the reaction rate in the living organism's metabolic pathways without itself getting altered is an enzyme. Most of the biological reactions and metabolic pathways in the living systems are carried out by enzymes. They are specific for their works and work in particular conditions. It maintains the best possible rate of reaction in the most stable state. The enzymes have distinct properties as they can proceed with the reaction in any direction, their particular binding sites, pH specificity, temperature specificity required in very few amounts.
If inhibitor X changes lactase activity to a Vo of 0.111111111111 mM per minute when [S] = 0.40
mM, and a Vo of 0.166666666667 mM per minute when [S] = 1.0 mM, calculate the new Vmax.
A. 0.10 mM per minute
B. 0.25 mM per minute
C. 0.50 mM per minute
D. 1.0 mM per minute
E. 2.0 mM per minute
Vmax, also known as the maximum velocity, is the maximum rate of an enzymatic reaction under optimal conditions when the enzyme is saturated with substrate. At Vmax, all enzyme molecules are bound to substrate, and any further increase in substrate concentration does not increase the rate of reaction.
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Calculate the slope on a Lineweaver-Burk plot (Km / Vmax) for the lactase reaction with inhibitor X.
A. 0.10 per minute
B. 0.25 per minute
C. 0.50 per minute
D. 1.0 per minute
E. 2.0 per minute
by Bartleby ExpertCalculate the new Km of the lactase reaction with inhibitor X.
A. 0.10 mM
B. 0.25 mM
C. 0.50 mM
D. 1.0 mM
E. 2.0 mM
by Bartleby Expert
