TLC, IR, MP TEMPLATE

Color BEFORE adding Color AFTER adding Intensity of color AFTER adding FeCl3 Test Tube # FeCl3 FeCl3 #1 (salicylic acid) clear lilac 100% #2 (commercial aspirin A) clear slightly pink 50% #3 (commercial aspirin B) clear lilac 95% #4 (aspirin from your clear pink 20% synthesis above) #5 (control) clear clear 0% Based on this data and the melting point of Aspirin, how pure is the synthesized product?

analyte concentration(C)(mg/ml) injection volume (ul) elution time (time) peak DAD signal(mAU) caffeine 1 1 4.67 302.85 aspartame 5 1 7.53 15.83 benzoic acid 1 1 8.14 89.98 saccharin 1 1 1.91 84.86 mixture(add everything above with 1:1:1:1 ratio)   1 4.47 69.58 How to get the concentration of the mixture in this case?

Gas chromatography is an analytical technique that can efficiently separate mixtures based on ________.     polarity     density     melting point     viscosity

What is the slope of the uncooked juice table attached?

. Experimental data mass (g) and volume (mL) of a substance was gathering, recorded, and plotted in Figure 1. a. Correctly la bel the graph axis (include units). b. The equation for the trendline (y = mx + b) is displayed on the graph. Which variable, in the equation, equals the density of the substance? A1 feMass B D Mass Volume 2. 1,8 2,19 Mass vs. volume 3 3,15 4,08 4,13 5,57 7. 6,7 8,34 6. y= 0,794x-0.064 8 6. 10 11. 2 4. 10 13 14 15 Figure 1: Plotting of Mass vs. Volume of a Substance. bttps://www.chemisalforums.com/index.php?toric=69575.0 Life University CHM 111 March 2021 Default Page Style English (USA)

1 and 2 answers?

0.2 to 1.0 mg/ml: For each concentration, a volume of 1.0 mL should be prepared. How will you prepare these standards using micropipettes and which one would you use? There is a: 0,5-10 microliter pipette; 2-20 microliter pipette 20-200 microliter pipette 100-1000 microliter pipette.

Table #2 Worktable for BCA Assay Standard Water (μl) Tube # 1 2 3 4 5 6 7 8 9 0 125 325 175 325 325 325 400 400 BSA source (μl) 300 stock 375 stock 325 stock 175 of tube #2 325 of tube #3 325 of tube #5 325 of tube #6 100 of tube #7 0 Protein (ug) Add the volumes listed in Table 2 to each tube. Add the water first and then the appropriate standard. Pipette each of the protein samples directly into the water, do not dribble on the side of the test tubes. The standard is at a concentration of 2 mg/ml and contains bovine serum albumin (BSA). Calculate the amount of BSA protein added to each of the tubes and write this in Table 2 (Protein (ug)).

Table 1: The result for test tube 1 to 8 using of BSA stock solution. Tube BSA Cone H;O (ml) BSA (ml) Biuret ABS mg/ml reagent (ml) 1. 1.0 4. 0. 2. 0.9 0.1 4. 0.003 3. 2. 0.8 0.2 4. 0.012 4. 3. 0.7 0.3 4. 0.021 5. 4. 0.6 0.4 4. 0.028 6. 0.5 0.5 4. 0.039 7. 6. 0.4 4. 0.042 8. 7. 0.3 0.7 4. 0.052 Table 2: The result for test tube 9 to 12 using of Unknown solution Protein Tube Uknown (ml) H;0 (ml) Biuret reagent ABS concentration (mg/ml) (ml) 9. 4. 0.036 10. 4. 0.035 11. 0.5 0.5 4. 0.017 12. 0.5 0.5 4 0.016 In order to determine the actual concentration of protein in the unknown samples it is necessary to draw the standard curve (concentration on the x-axis and on the absorbance y- axis) and interpolate the absorbance values of the unknowns. Draw graph and find protein concentration(mg/ml)

What is the chemical equation for main test and for the confirmation test?

Help Save & Exit Submit Methanol (CH3OH) is converted to bromomethane (CH3Br) as follows: CH3OH + HBr CH3B + H20 If 12.23 g of bromomethane are produced when 5.00 g of methanol is reacted with excess HBr, what is the percentage yield? Multiple Choice 12.9% 33.8% 59.1% 2:41 PM 11-Mar-2. asus COLLECTION prt sc delete 12/A 7 (3) & %3D 8.

This is not an assignment, trying to practice for my lab exam with similar questions that may be on there.

A piece of filter paper is placed in the TLC developing chamber to: Choose one: provide a lining for the TLC plate to rest on.   help the chamber saturate with the developing solvent's vapor.   make it easier to visualize the solvent front level.   protect the TLC plate.

Pls answer 3 & 4

The volume of the solution that can be measured with 5-10 microliter pipette and 2-20 microliter pipette is 0.005-0.01 mL and 0.002 mL-0.02 mL. The volume of the solution that can be measured with 20-200 microliter is 0.02-0.2 mL. The volume 1 mL of solution is measured with 100-100 microliter which has capacity of 0.1 mL to 1 mL. What would the display read for 0.10 mL, 0.20mL, 0.40mL, 0.60 mL, 1.0 mL read when using the micropipettes mentioned above

EXPERIMENT : Determination of Temporary and Permanent Hardness of Water .  I want Result & Analysis for this experiment includes the Precautions

If the two trials vary by more than 4 seconds, run a third trial and average the two closest times in seconds.   help me fill the table