C888 Task 2

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C888

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Jan 9, 2024

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KPP: Metabolic Processes PAGE 1 Metabolism C888-PRFA-KPP1~TASK 2 KELSEY CONKLIN
<Assessment Code: Task Title> <Attachment Title> A. Create an original model or a visual aid that demonstrates the function of an enzyme. 1. Describe a learning activity in which the model or visual aid from part A l could be used to facilitate student understanding of enzyme function. I would have my students fill out a diagram like mine and have them label them and then write a few sentences explaining what they see on my model and what the different parts are. After that we would have a group discussion on what they see. B. Create an experiment that would allow your students to assess how a changed condition would affect a selected type met a colic of process of an organism. Including the following in your experiment: 1. Identify the type of organism and the corresponding metabolic process you will be investigating in your experiment. 2. Hypothesize the effect of changing a condition on the metabolic process of the organism identified in part B 1. 3. Describe, in steps how the hypothesis proposed in part B2 could be investigated. Include each of the following elements: - Control sample - List of dependent, and independent variables - Material needed to complete investigation 4. Explain the metabolic reactions occurring in the control and experimental samples. PAGE 2
KPP: Metabolic Processes 5. Lab Template Organism or type of organism Rate of photosynthesis with leaf disk Metabolic process in the selected organism Spinach Leaves Hypothesis If the rate of photosynthesis is affected by the amount of time light is available, then the leaf disk that are under direct sunlight should have the highest rate of photosynthesis because they will have all of the reactants present. 6 C O 2 + 12 H 2 O + e --> 2 C 6 H 12 O 6 + 6 O 2 Control sample Sunlight Experimental sample No Sunlight (Dark Room) Dependent variable(s) Rate of Photosynthesis, measured with floating leaf disks. Independent variable(s) Different time amount of sunlight available Controlled variable(s) Heat, Humidity, water, Carbon Dioxide Materials Tin foil Covered Beaker 2 500 mL beakers containing 20mL of sodium bicarbonate solution 0.2% Sodium Bicarbonate solution Syringe Straw Spinach Leaves Steps to investigate hypothesis (procedure) 1.) This procedure is testing the rate of photosynthesis by showing how long it will take for a spinach leave to float back to the top after all the oxygen is removed. 2.) Use your straw to cut out ten leaf disks from the spinach leaves. You’re going to take the end of your straw and press it into the leaves. By using a twisting motion to cut them out. 3.) Remove the plunger from your syringe. Take the ten leaf disks and out them into the syringe. Make sure the leaves are at the bottom of the syringe and then replace the plunger. Make sure the plunger doesn’t damage any of your leaves. 4.) Carefully draw 10 mL of 0.2% sodium bicarbonate into the syringe. The leaves should be floating. The leaves are floating because of the oxygen that is being released due to photosynthesis. 5.) Press the plunger gently while facing the tip of the syringe up until all of the air is out of the syringe chamber. PAGE 0
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<Assessment Code: Task Title> <Attachment Title> 6.) Place your finger on the tip of the syringe and pull the plunger backwards like your taking it out but DO NOT pull it all the way out. This will create a vacuum inside the syringe. Hold it for about 10 seconds. Bubbles should be forming around the leaf disk. 7.) Release your finger and the plunger at the same time. The solution should rush into the air space in the leaf disk causing them to sink. The sink because there is no more oxygen left in the leaf disks. 8.) Once you get your leaves to sink then you must pour them into a cup with sodium Bicarbonate and set them under a light. Repeat steps 2-7 with adding the cups to different light intensities. 9.) The Cup with the most light had the fastest time of the leave disks to float again. While the other cups were slower. The cup with the lowest light intensity took the longest because there was not a lot of light which means as photosynthesis was happening cellular respiration was happing as well which was taking the oxygen from the cell which caused the leaf disk to take over 30 minutes for them to all be floating. Explanation of metabolic reactions in control and experimental samples In the Experimental group, we will be measuring how much oxygen becomes available without sunlight. In theory, without the photon of light, any reaction dependent on light can not happen because the catalyst is not available. In this group, we should not expect to see the majority of leaves floating by the end of the 14 mins because we are removing one of the reactants needed for photosynthesis. We know photosynthesis involves a reaction of carbon dioxide with water and light to produce oxygen and glucose. In the control group we will be measuring the production of oxygen with the available reactants. Light and water enter the chloroplast to start a reaction with ADP and NADP+ to produce ATP and NADPH. Oxygen being a by-product of this reaction. Because of this reaction we at the end of the 14 mins we should see majority if not all of the leaves floating. We should see this because all of the variables are present to start a reaction for photosynthesis. [ CITATION Exp \l 1033 ] C. Acknowledge sources, using in-text citations and references, for content that is quoted, paraphrased, or summarized. References Exploratorium. (n.d.). Photosynthetic Floatation . Retrieved from Exploratorium : https://www.exploratorium.edu/snacks/photosynthetic-floatation PAGE 1
<Assessment Code: Task Title> <Attachment Title> PAGE 2

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