Unit 1 quiz review
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Module 1 Quiz Review
Module 1 Quiz Review
You will see ONE of the following short answer questions on your quiz:
Even though the Germ theory had not yet been proven, why was Lister adamant about using sterile technique?
How does oil immersion improve resolution? – the question is telling you the resolution is improved, you need to tell me how it improves the resolution.
You performed a gram stain, but you were in a hurry and you forgot to add the counterstain, safranin. Tell me what color your gram positive and gram negative organisms would be and why.
– For the remaining quiz questions, you will need to know the gram stain technique, all of the reagents, what their functions are and the order they are used in. If you understand that you can easily answer this question.
The media reports that "new" infections seem to appear on a regular basis. What is the explanation for these infections? Are they "new"? - look this one up in the text. We are traveling more, encroaching on environments, disturbing ecological niches….
When antibiotics and vaccination techniques were first discovered, the statement was made that all pathogenic organisms would be controlled by the twenty-first century. Why do you think
this statement did not come true? Think about this one, why are we experiencing another pandemic?
In 1864, Lister observed that patients recovered from simple bone fractures but often had terrible complications with compound fractures. He knew application of phenol prevented cattle
disease so he treated all compound fractures with phenol and the patients began to recover without incident. How was Lister influenced by Pasteur and why was Koch's work still needed? Know the basic work of Lister, Pasteur and understand why Koch’s postulates were different from anything else done at the time.
In 1835 Bassi showed that a silkworm disease was caused by a fungus, and in 1865 Pasteur found another silkworm disease was caused by a protozoan. Why do we use Koch's postulates instead of "Bassi's" or "Pasteur's" postulates? – Know and understand what Koch’s postulates accomplished and why they were unique.
Describe why Pasteur's tipped flask experiment helped to disprove spontaneous generation. – Look up his experiment and understand it’s significance
When doing a gram stain, one step can be eliminated and still allow distinction between gram positive and gram negative organisms. What is the step and why would eliminating it still result in the ability to distinguish gram positive from gram negative? (As mentioned previously, you need to know and understand the technique and the reagents used.)
The following information will also help you in the remaining portion of the quiz:
Know the basic types of microscopes, how the objectives work to provide magnification, how oil immersion increases resolution, and basic components
Know Van Leeuwenhock was the first to observe microbes under a microscope
Know that opportunistic infections are those acquired by immunocompromised individuals
Know the terms aerobic and anaerobic
Know the basic difference between Eukaryotic, Prokaryotic, Archea prions and viruses
Understand normal flora and its functions
Understand bioremediation
Know the 3 Domain System and the types of cells that are represented
Know the cell theory
Know the term asepsis and Pasteurization
Know some of the benefits of microbes
Know some of the uses of biotechnology and some of the natural materials produced by microbes
Understand scientific notation, how do you know the genus versus the species name
Know simple staining, negative staining and their functions
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Related Questions
BONUS (15 points)
The fallowing series of dilution was prepared from a specimen to determine the number of bacteria.
There were 62 colonies on the agar plate prepared by transferring 0.3 ml from the tube number 4.
Calculate the dilution factors for each tube.
What is the cell concentration in the original specimen?
Calculate the total number of cells in tube number 2.
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A) F12
F9
F10
F7
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%3D
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The student advises using the same selection procedures and inoculating a 5 mL overnight culture with the transformed cells instead of searching for transformed coli on LB agar plates in order to save time. Why isn't this a wise decision?
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Why is it important to limit the quantity of cells used to prepare a smear?
Mark all that apply:
1. So that cells are not clumped and don't entrap stain creating erroneous results
2. So that the cells are spread out enough that cell morphology can be discerned
3. So that there are small groups of cells clumped together to make them visible
4. So that no contaminants are introduced onto the slide by being entrapped in clumps
5. So that the cells are spread out enough that the arrangement can be observed
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Can you help me?
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convert the procedure to passive voice and past tenses
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What is the purpose of fixing a smear?
Mark all that apply:
1. To attach the bacteria to the slide
2. To cause the cells to shrink and become distorted
3. To kill the bacteria so they aren't harmed by the staining method
4. To break down the cell wall in order to make the cells accept stain
5. To kill the bacteria to make the slide safer to handle
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PLEASE ANSWER THE FOLLOWING QUESTIONS ABOUT THE PICTURES.
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Your lazy labmate needs your help! They set up a 10-ml culture of the slow-growing Myxococcus
xanthus yesterday, but then they decided to stay up late playing videogames and won't be in until
next Monday to recover. Whoops! They call you in lab that Friday and ask you to take an OD
reading for them. You do this favor for them, and helpfully do the conversion into cells/mL,
finding that their culture is currently sitting at about 3.0 x108 cells per mL. Your labmate is
horrified: they won't be back in time before the cells hit stationary phase, since M. xanthus
doubles every 5 hours. They beg you to make a new culture using these cells and set it up to
reach the same cell density Monday morning. That's 59 hours away. If the cells usually lag for
three hours upon dilution into fresh medium, what cell density should you dilute the culture to?
About 1.3 x 107 cells/ml
About 1.3 x 105 cells/ml
About 1.3 x 106 cells/ml
About 1.3 x 104 cells/ml
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1. Describe the three different type of hemolysis that are observed on blood agar.2. What is a selective medium?3. What is a differential medium?4. Which media can be used to isolate E. coli samples from contaminated lettuce?5. Which two media would be used to identify a sample taken from a patient with suspected gonorrhea?6. Would you be able to grow a sample obtained from a patient's wound (suspected to be infected with MRSA) on EMB? Explain.7. What is the color or TSI for Salmonella?8. What is a fastidious organism?
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What part of the tube changes color first (in Resazurin Reduction Test between Pasteurized and Raw Milk)?
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I need help with a micobiology question, Explain why adding 1 ml of a phage sample into a 9 ml tube of sterile saline creates a 1:10 dilution?
thank you
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A student needed to transfer bacteria from a broth culture to an agar plate. Below is the step-by-step what was done to accomplish this. The transfer of bacteria was not successful because of which step? 1. Cap of the broth culture is removed 2. The mouth of the bottle is flamed 3. The loop was flamed 4. The loop was inserted into the culture to pick up the bacteria 5. The loop was flamed 6. The loop containing the bacteria was used to introduced to spread the agar plate 7. The plate was placed in an incubator at 30 Celcius
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I need help ! I don’t know if this is correct
- staphylococcus aureus-
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I am wondering whether E. coli can be used as spiked in sample for Bacteria vaginosis detection through qPCR? Please help me with the information including how to purchase it.
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In order to do electron microscopy the samples had to be specially prepared. Were the cells alive at the time of viewing? Explain why you said yes or no
I need help answering this queshtion the answer is with the article and it has to be as short as possible
URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC106848/
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Show work want correct answers ASAP
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Question:-
How to perform a bactericidal test bactericidal test of Dental Pulp Stem Cell (DPSC)?
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Please help a bit confused
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List out steps on how to properly clean a traceostomy tube
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How do I know what errors would effect my result in gram staining experiments?
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Hi, I want to ask the question related to antibiotics resistance? The question is the non-existance of solidifying agent may produce a liquid like culture media. Name the method used to determine the Antibiotic Susceptibility test which uses the said media? Can explain answer? Thank you.
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I AM TRYING TO IDENTIFY THIS UNKNOWN.
***IMAGE 1 HAS TWO PICTURE OF CATALASE TEST AND BLOOD AGAR TEST.
***IMAGE 2 HAS TWO TESTS CONDUCTED ON IT, BACITRACIN AND PYR
I believe it is one of the following:
1) S. pyo.
2)S. agal .
3)S.pneu.
4)E. faecalis
5)S. aureus
6)S epi.
7)S. sapro.
8)M. luteus
Please let me know which one of the above is thge unknown. You have pictures of the unkown on Catalase and Blood Agar and three test conducted on Bacitracin and PYR.
Give reasoning as why you think it will be one of the above. Explaing the characteristics and what made you deicide it?
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Need help filling this out
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LABORATORY REPORT: Aseptic Technique & Inoculation of Bacteria
Guide Questions.
1. Why is direct flaming preferred when disinfecting loops and needles?
2. Why is it important to flame the entirety of the loop and not just the tip? What consequences can be seen when this process is not done correctly?
3. What is the difference between quadrant streak method A from method B?
4. Why do we use a pencil instead of a pen when labelling culture tubes or plates? Are there other alternatives in labelling?
5. Why is an inoculating needle used in Subculturing Techniques? Can a loop be used instead?
NOTE: Please try to answer all of the question asked, i promised to give you a good ratings
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136. A 17-year-old boy who is sexually active has a 9-day history of several necrotic pustules on his arms and legs and tenosynovitis of his wrist, and a 2-day history of swelling and pain in his
right knee. Aspiration of the knee joint is positive for microorganisms on Gram stain. Which of the following findings on Gram staining best describes the pathogen most likely causing this
patient's condition?
induced oskowglitis
A) Gram-negative, bipolar-staining rods
B) Gram-negative, kidney-bean-shaped cocci
C) Gram-positive-cocci in chains-
D) Gram-positive cocci in clusters
E) Pleomorphic gram-negative coccobacilli
the
gemorhe
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Related Questions
- BONUS (15 points) The fallowing series of dilution was prepared from a specimen to determine the number of bacteria. There were 62 colonies on the agar plate prepared by transferring 0.3 ml from the tube number 4. Calculate the dilution factors for each tube. What is the cell concentration in the original specimen? Calculate the total number of cells in tube number 2. étv A) F12 F9 F10 F7 % & %3D delete 5 { T Y J Karrow_forwardThe student advises using the same selection procedures and inoculating a 5 mL overnight culture with the transformed cells instead of searching for transformed coli on LB agar plates in order to save time. Why isn't this a wise decision?arrow_forwardWhy is it important to limit the quantity of cells used to prepare a smear? Mark all that apply: 1. So that cells are not clumped and don't entrap stain creating erroneous results 2. So that the cells are spread out enough that cell morphology can be discerned 3. So that there are small groups of cells clumped together to make them visible 4. So that no contaminants are introduced onto the slide by being entrapped in clumps 5. So that the cells are spread out enough that the arrangement can be observedarrow_forward
- Can you help me?arrow_forwardconvert the procedure to passive voice and past tensesarrow_forwardWhat is the purpose of fixing a smear? Mark all that apply: 1. To attach the bacteria to the slide 2. To cause the cells to shrink and become distorted 3. To kill the bacteria so they aren't harmed by the staining method 4. To break down the cell wall in order to make the cells accept stain 5. To kill the bacteria to make the slide safer to handlearrow_forward
- PLEASE ANSWER THE FOLLOWING QUESTIONS ABOUT THE PICTURES.arrow_forwardYour lazy labmate needs your help! They set up a 10-ml culture of the slow-growing Myxococcus xanthus yesterday, but then they decided to stay up late playing videogames and won't be in until next Monday to recover. Whoops! They call you in lab that Friday and ask you to take an OD reading for them. You do this favor for them, and helpfully do the conversion into cells/mL, finding that their culture is currently sitting at about 3.0 x108 cells per mL. Your labmate is horrified: they won't be back in time before the cells hit stationary phase, since M. xanthus doubles every 5 hours. They beg you to make a new culture using these cells and set it up to reach the same cell density Monday morning. That's 59 hours away. If the cells usually lag for three hours upon dilution into fresh medium, what cell density should you dilute the culture to? About 1.3 x 107 cells/ml About 1.3 x 105 cells/ml About 1.3 x 106 cells/ml About 1.3 x 104 cells/mlarrow_forward1. Describe the three different type of hemolysis that are observed on blood agar.2. What is a selective medium?3. What is a differential medium?4. Which media can be used to isolate E. coli samples from contaminated lettuce?5. Which two media would be used to identify a sample taken from a patient with suspected gonorrhea?6. Would you be able to grow a sample obtained from a patient's wound (suspected to be infected with MRSA) on EMB? Explain.7. What is the color or TSI for Salmonella?8. What is a fastidious organism?arrow_forward
- What part of the tube changes color first (in Resazurin Reduction Test between Pasteurized and Raw Milk)?arrow_forwardI need help with a micobiology question, Explain why adding 1 ml of a phage sample into a 9 ml tube of sterile saline creates a 1:10 dilution? thank youarrow_forwardA student needed to transfer bacteria from a broth culture to an agar plate. Below is the step-by-step what was done to accomplish this. The transfer of bacteria was not successful because of which step? 1. Cap of the broth culture is removed 2. The mouth of the bottle is flamed 3. The loop was flamed 4. The loop was inserted into the culture to pick up the bacteria 5. The loop was flamed 6. The loop containing the bacteria was used to introduced to spread the agar plate 7. The plate was placed in an incubator at 30 Celciusarrow_forward
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