Chapter12: Parenteral Equipment And Supplies
Section: Chapter Questions
Problem 22RQ
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Question
convert the procedure to passive voice and past tenses
![Effectiveness of sunscreens against UV radiation on Bacterial growth
1. Label the bottom of your four Petri dishes with your group name and date. Divide the
plate in half by drawing a line on the bottom haif of the plate (part with agar). Label
one side "C" for control and the other side with sunscreen and SPF.
2. Figure I illustrates how to adapt the dilution and plating procedure. Prepare a dilution
series of bacteria.
O1 ml. 01 ml. 01 ml.
Ixto.
Cells/ml. 0
9ml Pml.
Oml.
oml.
eml.
ml.
Ixto Cells Ixtocells Ixto Cells
Figure 1.
3. While holding the lid to avoid contamination, pipette 0.1 ml. of bacteria from the
sample into each dish. Using the bacteria spreader, spread the sample evenly
throughout the entire dish. Or using a sterile cotton applicator, streak innoculate a
nutrient agar plate to form a bacterial lawn over the entire surface. Repeat this
process for all remaining plates.
4. Cut plastic wrap large enough to cover the top of cach Petri dish. Remove the lid and
cover the top of the Petri dish with the plastic wrap. Snuggly fold the edges beneath
the bottom of the Petri dish. Secure the edges of the plastic wrap with masking tape.
Keep the Petri dish lids for later use.
5. Weigh out 0.5 grams of sunscreen for each Petri dish. Apply the sunscreen evenly to
the plastic wrap using the same technique for cach sunscreen.
6. Cut and fold in place pieces of aluminum foil large enough to cover the control half
of each Petri dish.
7. Using your UV light source (can be sunlight), expose your Petri dishes to the light for
three minutes making sure dishes are same distance from light source.
8. Once samples have been exposed to UV light, remove the aluminum foil and plastic
wrap. Put the Petri dish lids back on.
9. Stack your plates into groups of four and tape them together with the masking tape.
Make sure your group name is clearly written on the masking tape. Place the dishes,
inverted, into the incubators (25° C)](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fd1fe35ba-9522-4ff0-b531-dfff3af7ea47%2Fbc4663fd-4552-4a81-889a-0bab9cfbf91e%2F7sdqrwh_processed.jpeg&w=3840&q=75)
Transcribed Image Text:Effectiveness of sunscreens against UV radiation on Bacterial growth
1. Label the bottom of your four Petri dishes with your group name and date. Divide the
plate in half by drawing a line on the bottom haif of the plate (part with agar). Label
one side "C" for control and the other side with sunscreen and SPF.
2. Figure I illustrates how to adapt the dilution and plating procedure. Prepare a dilution
series of bacteria.
O1 ml. 01 ml. 01 ml.
Ixto.
Cells/ml. 0
9ml Pml.
Oml.
oml.
eml.
ml.
Ixto Cells Ixtocells Ixto Cells
Figure 1.
3. While holding the lid to avoid contamination, pipette 0.1 ml. of bacteria from the
sample into each dish. Using the bacteria spreader, spread the sample evenly
throughout the entire dish. Or using a sterile cotton applicator, streak innoculate a
nutrient agar plate to form a bacterial lawn over the entire surface. Repeat this
process for all remaining plates.
4. Cut plastic wrap large enough to cover the top of cach Petri dish. Remove the lid and
cover the top of the Petri dish with the plastic wrap. Snuggly fold the edges beneath
the bottom of the Petri dish. Secure the edges of the plastic wrap with masking tape.
Keep the Petri dish lids for later use.
5. Weigh out 0.5 grams of sunscreen for each Petri dish. Apply the sunscreen evenly to
the plastic wrap using the same technique for cach sunscreen.
6. Cut and fold in place pieces of aluminum foil large enough to cover the control half
of each Petri dish.
7. Using your UV light source (can be sunlight), expose your Petri dishes to the light for
three minutes making sure dishes are same distance from light source.
8. Once samples have been exposed to UV light, remove the aluminum foil and plastic
wrap. Put the Petri dish lids back on.
9. Stack your plates into groups of four and tape them together with the masking tape.
Make sure your group name is clearly written on the masking tape. Place the dishes,
inverted, into the incubators (25° C)
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