BILD 1 Week 5 section materials (asynch)

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UCSD BILD 1: The Cell Section, Week 5 UCSD BILD 1: The Cell Question 1 This is a graph of the rate of reaction vs. the pH for the protein pepsin, an enzyme that breaks down protein in the human stomach. The human stomach is very acidic (low pH). Another protein that breaks down protein, trypsin, is in the human small intestine. The pH of the small intestine is slightly basic, around pH 8. On the same graph, please draw the predicted rate of reaction vs. pH for trypsin. Question 2 Your study friend tells you, “ The oxygen you inhale does not automatically become part of your dry biomass. ” Would you agree or disagree with this statement, and why? (suggestion: 1-3 sentences) Question 3: A man who worked is experiencing an illness with many symptoms, including muscle weakness. His mother alleges that his wife is poisoning him, and you are assigned to investigate. A doctor’s visit shows mitochondrial damage. Levels of key molecules are as follows: a. You learn that the wife’s parents used to be woodworkers, so in their old shed, she has access to arsenate, which used to be used to preserve wood until it was banned for being toxic. Arsenate inhibits glycolysis. Are the medical results consistent with arsenate poisoning? Justify your reasoning. (roughly 1-2 sentences) Molecule Level compared to normal Pyruvate High Acetyl Co-A Low NADH Low Rate of reaction à
UCSD BILD 1: The Cell Section, Week 5 UCSD BILD 1: The Cell b. The man missed his childhood home, and he had his mother ship him water from their well, which he drinks almost exclusively. (His mother only drinks Coke.) You find that the water has naturally very high levels of arsenite, which mainly inhibits the transition reaction. Are the medical results consistent with arsenite poisoning? Justify your reasoning. (roughly 1-2 sentences) c. In his job, the man used to spread sodium fluoroacetate to combat mice. Sodium fluoroacetate only inhibits the citric acid cycle. Are the medical results consistent with sodium fluoroacetate poisoning? Justify your reasoning. (roughly 1-2 sentences) d. The couple previously had a flea problem in their house, so the wife had access to a store of the pesticide rotenone. Rotenone only inhibits the electron transport chain. Are the medical results consistent with rotenone poisoning? Justify your reasoning. (roughly 1-2 sentences)
UCSD BILD 1: The Cell Section, Week 5 UCSD BILD 1: The Cell Question 4: The active site of enolase contains a glutamate (shown below). a. If we replace the glutamate with one of the amino acids below, which substitutions might allow enolase to remain functional? Circle all that are correct. Amino acid Structure Functional? (write yes or no) Glycine Aspartate Serine Threonine Histidine b. Justify your response; include your reasoning for the ones you chose and the ones you did not. (roughly 1-2 sentences)
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