Lab 4 S22

Megan McCoy Bio 216-010 Lab 4 Diffusion, Osmosis, and Tonicity Write Up Experiment 1 1. Design a table to represent your own group data and paste it below. Include a figure caption. (1) Table 1. Distance dye has traveled in agar filled test tubes over 90 minutes, for the single table group. With distance measured in millimeters. Minutes Passed 0 10 20 30 40 50 60 70 80 90 Dye 0.10 M Potassium Permanganate (MW 158 g/mol) 0 5 6 8 8 10 11 12 14 14 0.10 M Methylene Blue (MW 374 g/mol) 0 1 2 3 3 3 4 4 4 4 0.01 M Methylene Blue (MW 374 g/mol) 0 1 1 2 2 2 3 3 3 3 0.10 M Congo Red (MW 697 g/mol) 0 2 4 4 5 5 5 6 6 6 2. Design a table to represent the class data (from the shared spreadsheet) and paste it below. Include a figure caption. (1) Table 2. Distance dye has traveled in agar filled test tubes over 90 minutes, using class averages. With distance measured in millimeters. Minutes Passed 0 10 20 30 40 50 60 70 80 90 Dye 0.10 M Potassium Permanganat e (MW 158 g/mol) 0 6.80 7.83 9.50 10.1 7 11.5 0 12.8 3 13.4 2 14.3 3 15.3 3

0.10 M Methylene Blue (MW 374 g/mol) 0 1.92 2.50 3.08 3.17 3.42 3.72 3.75 3.92 4.00 0.01 M Methylene Blue (MW 374 g/mol) 0 1.50 2.00 2.25 2.25 2.25 2.50 2.67 2.67 2.75 0.10 M Congo Red (MW 697 g/mol) 0 3.42 4.58 4.92 5.33 5.50 5.85 6.08 6.50 6.92 3. Produce a line graph that reports the average value for the class. Your graph should include A figure caption, figure legend, axis labels, and standard deviation error bars. (1) Figure 1. Average distance traveled through agar-filled test tubes in four dye types for 90 minutes. The 0.10 M Potassium Permanganate traveled the farthest compared to the other dyes. The distance traveled was measured in millimeters every 10 minutes for 90 minutes, watching as the dye traveled downward through agar-filled test tubes. Each table group collected data on the dye’s travel distance, and averages were calculated and used to construct the graph. 4. What conclusions can you draw from the diffusion experiment? Be sure to address at least two factors which affect the rate of diffusion. (1) The experiment dealt with changes in concentration and solute masses. The dye that traveled

Figure 2. Red blood cell in hypotonic solution of 0.0% NaCl at x1000 oil objective. Figure 3. Red blood cell in isotonic solution of 0.3% NaCl at x1000 oil objective. Figure 4. Red blood cell in isotonic solution of 0.85% NaCl at x1000 oil objective. 3.5% NaCl Figure 5. Red blood cell in hypertonic solution of 3.5% NaCl at x1000 oil objective. 10.0% NaCl Figure 6. Red blood cell in hypertonic solution of 10.0% NaCl at x1000 oil objective. 9. Is there a % NaCl solution that would be physiologically appropriate for animal cells? Why would one want to know the answer to this question? (1) An appropriate %NaCl solution for animals cells exists. This would provide information on what environment cells thrive in and what would be detrimental. An environment that is unsuitable to the cells would cause immense stress and greatly impact the health of the organism. In this circumstance, the sheep cells did well in the 0.85% NaCl solution which was isotonic, causing minimal and not damaging osmosis.