Solutions for FUNDAMENTALS OF BIOCHEMISTRY WPNG 1-SEME
Problem 4E:
4. Protein X has an absorptivity of 0.4 mL · mg?1 · cm?1 at 280 nm. What is the absorbance at 280 nm...Problem 5E:
5. You are using ammonium sulfate to purify protein Q (pI = 5.0) by sailing out from a solution at...Problem 7E:
7. (a) In what order would the amino acids Arg, His, and Leu be eluted from a carboxymethyl column...Problem 9E:
9. Explain why a certain protein has an apparent molecular mass of 90 kD when determined by gel...Problem 10E:
10. Determine the subunit composition of a protein from the following information:
Molecular mass by...Problem 11E:
11. Explain why a protein, which has a sedimentation coefficient of 2.6S when ultracentrifuged in a...Problem 12E:
12. A protein has an apparent mass of 800 kD by gel filtration chromatography, but SDS-PAGE shows a...Problem 13E:
13. Explain why the dansyl chloride treatment of a single polypeptide chain followed by its complete...Problem 14E:
14. Identify the tint residue obtained by Edman degradation of cytochrome c from (a) Drosophila, (b)...Problem 15E:
15. A pentapeptide has the sequence NNKNN (using one-letter symbols for amino acids). Calculate the...Problem 16E:
16. The peptide in Problem 15 was sequenced by tandem mass spectrometry. What are the expected...Problem 17E:
17. In site-directed mutagenesis experiments, Gly is often successfully substituted for Val, but Val...Problem 18E:
18. Below is a list of the first 10 residues of the B helix in myoglobin from different...Problem 23CQ:
23. What fractionation procedure could be used to purify protein 1 from a mixture of three proteins...Problem 25CQ:
25. Purification tables are often used to keep track of the yield and purification of a protein. The...Problem 27CQ:
27. You must cleave the following peptide into smaller fragments. Which of the proteases listed in...Problem 28CQ:
28. You wish to determine the sequence of a polypeptide that has die following amino acid...Problem 31CQ:
31. You wish to determine the sequence of a short peptide Cleavage with trypsin yields three smaller...Browse All Chapters of This Textbook
Chapter 1 - Introduction To The Chemistry Of LifeChapter 2 - WaterChapter 3 - Nucleotides, Nucleic Acids, And Genetic InformationChapter 4 - Amino AcidsChapter 5 - Proteins: Primary StructureChapter 6 - Proteins: Three-dimensional StructureChapter 7 - Protein Function: Myoglobin And Hemoglobin, Muscle Contraction, And AntibodiesChapter 8 - CarbohydratesChapter 9 - Lipids And Biological MembranesChapter 10 - Membrane Transport
Chapter 11 - Enzymatic CatalysisChapter 12 - Enzyme Kinetics, Inhibition, And ControlChapter 13 - Biochemical SignalingChapter 14 - Introduction To MetabolismChapter 15 - Glucose CatabolismChapter 16 - Glycogen Metabolism And GluconeogenesisChapter 17 - Citric Acid CycleChapter 18 - Electron Transport And Oxidative PhosphorylationChapter 19 - PhotosynthesisChapter 20 - Lipid MetabolismChapter 21 - Amino Acid MetabolismChapter 22 - Mammalian Fuel Metabolism: Integration And RegulationChapter 23 - Nucleotide MetabolismChapter 24 - Nucleic Acid StructureChapter 25 - Dna Replication, Repair, And RecombinationChapter 26 - Transcription And Rna ProcessingChapter 27 - Protein SynthesisChapter 28 - Regulation Of Gene Expression
Sample Solutions for this Textbook
We offer sample solutions for FUNDAMENTALS OF BIOCHEMISTRY WPNG 1-SEME homework problems. See examples below:
Functional groups are the reactive regions of molecules that are functionalized with these specific...Pictorial presentation: Fig. 1 shows structure of guanine, where the potential hydrogen bond donors...Explanation: The given structure in the diagram is of the nucleoside diphosphate called guanosine...Amino acid is a basic structural unit of a protein. It forms a peptide bond by joining two or more...Explanation: The sequence of amino acids forms primary structure that are linked together by peptide...Fig. 1 shows the cis peptide with groups that experience steric effects. The cis peptide bonds are...Explanation: When fractional saturation is half, it means that the ligand has occupied half of the...Explanation: The formula to calculate the number of stereoisomers in a given carbohydrate is 2n,...Explanation: Trans isomers have a higher melting point compared to cis isomers. It is mainly because...
Explanation: The extracellular concentration of glucose is 5mM = 0.005M. The intracellular...Reason for the correct statement: Option b. is given as, “It increases the rate at which a chemical...Calculation: Given: The nonenzymatic elementary reaction is AàB, where A is the reactant and B is...Hormones are of two types, namely, steroid hormones and peptide hormones. Steroid hormones are lipid...Explanation: Autotrophs possess the capability of synthesizing micronutrients including vitamins and...In glycolysis, 10 steps are involved to convert glucose to pyruvate; out of these, during four...Explanation: Glycogen is broken down when the cell needs to produce ATP from glucose. The enzyme...Explanation: Citric acid cycle is central to the metabolism. Acetyl CoA enters the citric acid cycle...Explanation: The mitochondrion is a cell organelle with outer and inner membrane made of lipid...In plants, the light reactions use light energy to produce adenosine triphosphate (ATP) and...Explanation: The enzyme glycerol kinase acts on the L-glycerol and it forms L-glycerol-3-phosphate....Explanation: ATP is the energy currency of the cell which can be renewed. In a cell when catabolism...The lower air pressure in the high altitudes makes it more difficult for oxygen to enter the...Explanation: The reaction in which aspartate is added to the IMP and adenylosuccinate lyase take...Explanation: Hypoxanthine is a naturally occurring modified form of purine base. Hypoxanthine is...Calculation: Given: The total genome content of E.coli chromosome=4×106 base pairs. The length of...Explanation: Cordycepin is also known as 3ʹ-deoxyadenosine. Adenosine is a cellular purine...Explanation: A 4-nt insertion is the addition of four nucleotides to the sequence. This means that...Explanation: The percentage of single-stranded DNA is plotted as a function of Cot; that is, the...
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