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Chapter 9, Problem 9.9P

Beef catalase has been used to accelerate the decomposition of hydrogen peroxide to yield water and oxygen [Chem. Eng. Educ., 5, 141 (1971)]. The concentration of hydrogen peroxide is given as a function of time for a reaction mixture with a pH of 6.76 maintained at 30°C.

Chapter 9, Problem 9.9P, Beef catalase has been used to accelerate the decomposition of hydrogen peroxide to yield water and

  1. (a) Determine the Michaelis–Menten parameters Vmax and KM. (Ans.: KM = 0.031 mol/dm3)
  2. (b) If the total enzyme concentration is tripled, what will the substrate concentration be after 20 minutes?
  3. (c) Apply one or more of the six ideas in Preface Table P-4, page xxviii, to this problem.
  4. (d) List ways you can work this problem incorrectly.
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